General Stability | Organism |
---|---|
bovine serum albumin stabilizes dilute enzyme solutions | Bos taurus |
Triton X-100 or bovine serum albumin enhances recovery during purification | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | complete inhibition at 0.1%, 1% Triton X-100 partially reverses | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | 0.002 | 1D-myo-inositol 1,4,5-trisphosphate | pH 7.5, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 6-7fold stimulation by Ca2+/calmodulin | Bos taurus | |
Ca2+ | activation by Ca2+/calmodulin | Bos taurus | |
Ca2+ | not activated by free Ca2+ | Bos taurus | |
Ca2+ | mechanism of activation | Bos taurus | |
Mg2+ | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE | Bos taurus |
38000 | - |
1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE | Bos taurus |
44000 | - |
gel filtration, in presence of Triton X-100 | Bos taurus |
52000 | - |
1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Bos taurus | target enzyme of cellular regulation, enzyme controls the rate of InsP3 metabolism | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
27800fold | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | 20 | pH 7.5, 37°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Bos taurus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | target enzyme of cellular regulation, enzyme controls the rate of InsP3 metabolism | Bos taurus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP-dependent | Bos taurus | |
Calmodulin | 6-7fold stimulation by Ca2+/calmodulin | Bos taurus | |
Calmodulin | activation by Ca2+/calmodulin | Bos taurus | |
Calmodulin | mechanism of activation | Bos taurus |