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Literature summary for 2.7.1.127 extracted from

  • Takazawa, K.; Passareiro, H.; Dumont, J.E.; Erneux, C.
    Purification of bovine brain inositol 1,4,5-trisphosphate 3-kinase. Identification of the enzyme by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis (1989), Biochem. J., 261, 483-488.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
bovine serum albumin stabilizes dilute enzyme solutions Bos taurus
Triton X-100 or bovine serum albumin enhances recovery during purification Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
SDS complete inhibition at 0.1%, 1% Triton X-100 partially reverses Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001 0.002 1D-myo-inositol 1,4,5-trisphosphate pH 7.5, 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Bos taurus 5829
-
soluble
-
Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 6-7fold stimulation by Ca2+/calmodulin Bos taurus
Ca2+ activation by Ca2+/calmodulin Bos taurus
Ca2+ not activated by free Ca2+ Bos taurus
Ca2+ mechanism of activation Bos taurus
Mg2+
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE Bos taurus
38000
-
1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE Bos taurus
44000
-
gel filtration, in presence of Triton X-100 Bos taurus
52000
-
1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Bos taurus target enzyme of cellular regulation, enzyme controls the rate of InsP3 metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
27800fold Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10 20 pH 7.5, 37°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
Bos taurus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate target enzyme of cellular regulation, enzyme controls the rate of InsP3 metabolism Bos taurus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52000, 1 * 38000, 1 * 35000, 3 bands with InsP3 3-kinase activity: isoenzymes or degradation products, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP ATP-dependent Bos taurus
Calmodulin 6-7fold stimulation by Ca2+/calmodulin Bos taurus
Calmodulin activation by Ca2+/calmodulin Bos taurus
Calmodulin mechanism of activation Bos taurus