Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by Triton X-100 | Homo sapiens |
General Stability | Organism |
---|---|
enzyme is more stable in Tris buffer than in potassium phosphate buffer | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at higher concentrations | Homo sapiens | |
EGTA | Ca2+/calmodulin-activated enzyme | Homo sapiens | |
Protein kinase C | phosphorylates and inactivates enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00076 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at physiological concentrations | Homo sapiens | |
Ca2+ | activation by Ca2+/calmodulin | Homo sapiens | |
Ca2+ | mechanism of activation | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, additional 53 kDa polypeptide with enzyme activity, SDS-PAGE | Homo sapiens |
58000 | - |
minor peaks with MW of 39 kDa and 32 kDa, HPLC gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens | inositol 1,4,5-trisphosphate is a second messenger | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens | key enzyme in inositol phosphate signaling pathway | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
943fold | Homo sapiens |
calmodulin-Sepharose affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
platelet | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.283 | - |
pH 7.4, 37°C | Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris-HCl, pH 7.5, 1 mM EGTA, 3 mM MgCl2, 1 mM DTT, 10% v/v glycerol, several months, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol 1,4,5-trisphosphate is a second messenger | Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | key enzyme in inositol phosphate signaling pathway | Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, additional 53 kDa polypeptide with enzyme activity, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
in presence of Ca2+/calmodulin | Homo sapiens |
7.4 | - |
assay at | Homo sapiens |
7.6 | - |
in presence of EGTA | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 7.6 | about half-maximal activity at pH 6.3 and 7.6 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
Calmodulin | activation by Ca2+/calmodulin | Homo sapiens | |
Calmodulin | Ca2+/calmodulin-dependent, 10fold stimulation, reversed upon chelation of Ca2+ by EGTA | Homo sapiens | |
Calmodulin | mechanism of activation | Homo sapiens |