Any feedback?
Literature summary for 2.7.1.127 extracted from
- Purification and biochemical properties of a high-molecular-mass inositol 1,4,5-trisphosphate 3-kinase isoenzyme in human platelets (1994), Biochem. J., 298, 669-673.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| calpain inhibitors stabilize during purification | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.4, 37°C, platelet enzyme | Homo sapiens |  |
| 2.5 | - |
ATP | pH 7.4, 37°C, platelet enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation by Ca2+/calmodulin | Homo sapiens | |
| Ca2+ | 17fold stimulation by Ca2+/calmodulin at saturating calmodulin and 0.01 mM free Ca2+ | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
3 isoenzymes: A and B, brain, and high-molecular weight platelet isoenzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4662fold | Homo sapiens |
| calmodulin-Sepharose affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| platelet | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.75 | 1.45 | pH 7.4, 37°C, platelet, in presence of free Ca2+ and calmodulin | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 69000-70000, platelet, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| Calmodulin | activation by Ca2+/calmodulin | Homo sapiens | |
| Calmodulin | 17fold stimulation by Ca2+/calmodulin at saturating calmodulin and 0.01 mM free Ca2+ | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
