Literature summary for 2.7.1.119 extracted from
Li, D.; Tang, N.; Liu, M.; Shen, C.; Hu, Y.; Xia, Y.; Cao, M.
Using hygromycin phosphotransferase and enhanced green fluorescent protein genes for tracking plastid transformation in rice (Oryza sativa L.) via gold particle bombardment (2016), Nanosci. Nanotechnol. Lett., 8, 409-417 .
No PubMed abstract available
Application
Application |
Comment |
Organism |
biotechnology |
use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice (Oryza sativa) |
Streptomyces sp. |
molecular biology |
use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice (Oryza sativa) |
Streptomyces sp. |
Cloned(Commentary)
Cloned (Comment) |
Organism |
recombinant gene expression in transgenic Oryza sativa from site-specific transformation vector of rice plastid, pCTE04 |
Streptomyces sp. |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice. A site-specific transformation vector of rice plastid, pCTE04, is constructed for dicistronic expression of the hygromycin phosphotransferase (hpt) and enhanced green fluorescent protein genes (EGFP). Transplastomic rice plants successfully grow up after a gradual selection of hygromycin resistance. Integration of the dicistronic expression cassette into the intergenic region trnI-trnA of plastid genome is verified by Southern blotting analysis. Transplastomic expression of the hpt and EGFP genes is identified by RT-PCR. The stable expression of dicistronic cassette in the rice is confirmed by confocal microscopy |
Streptomyces sp. |
Organism
Organism |
UniProt |
Comment |
Textmining |
Streptomyces sp. |
- |
- |
- |
Synonyms
Synonyms |
Comment |
Organism |
hygromycin phosphotransferase |
- |
Streptomyces sp. |