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Literature summary for 2.7.1.119 extracted from

  • Pardo, J.M.; Malpartida, F.; Rico, M.; Jimenez, A.
    Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus - the producing organism (1985), J. Gen. Microbiol., 131, 1289-1298.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
hygromycin B above 0.01 mM, substrate inhibition Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Streptomyces hygroscopicus
0.0015
-
hygromycin B pH 7.5, 30°C Streptomyces hygroscopicus
0.076
-
ATP pH 7.5, 30°C Streptomyces hygroscopicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + hygromycin B Streptomyces hygroscopicus resistance to hygromycin B also occurs in Escherichia coli, where a plasmid encodes a hygromycin B phosphotransferase activity that produces 4-phosphoryl-hygromycin B ADP + 7''-O-phosphohygromycin B
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Streptomyces hygroscopicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
-
Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + hygromycin B
-
Streptomyces hygroscopicus ADP + 7''-O-phosphohygromycin B
-
?
ATP + hygromycin B resistance to hygromycin B also occurs in Escherichia coli, where a plasmid encodes a hygromycin B phosphotransferase activity that produces 4-phosphoryl-hygromycin B Streptomyces hygroscopicus ADP + 7''-O-phosphohygromycin B
-
?