General Stability | Organism |
---|---|
glycerol and bovine serum albumin stabilize | Streptomyces hygroscopicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hygromycin B | above 0.01 mM, substrate inhibition | Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00056 | - |
hygromycin B | - |
Streptomyces hygroscopicus | |
0.0364 | - |
ATP | - |
Streptomyces hygroscopicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Streptomyces hygroscopicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement, optimal activity at 0.1 mM | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, SDS-PAGE | Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + hygromycin B | Streptomyces hygroscopicus | responsible for resistance against hygromycin B, an aminocyclitol antibiotic | ADP + 7''-O-phosphohygromycin B | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces hygroscopicus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
- |
Streptomyces hygroscopicus |
Storage Stability | Organism |
---|---|
-20°C, in 50% v/v glycerol and 0.1% bovine serum albumin, stable for up to 6 months | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + hygromycin B | - |
Streptomyces hygroscopicus | ADP + 7''-O-phosphohygromycin B | - |
? | |
ATP + hygromycin B | responsible for resistance against hygromycin B, an aminocyclitol antibiotic | Streptomyces hygroscopicus | ADP + 7''-O-phosphohygromycin B | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Streptomyces hygroscopicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces hygroscopicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.5 | pH 6.6: about 40% of maximal activity, pH 8.5: about 65% of maximal activity | Streptomyces hygroscopicus |