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Literature summary for 2.7.1.119 extracted from

  • Zalacain, M.; Pardo, J.M.; Jimenez, A.
    Purification and characterization of a hygromycin B phosphotransferase from Streptomyces hygroscopicus (1987), Eur. J. Biochem., 162, 419-422.
    View publication on PubMed

General Stability

General Stability Organism
glycerol and bovine serum albumin stabilize Streptomyces hygroscopicus

Inhibitors

Inhibitors Comment Organism Structure
hygromycin B above 0.01 mM, substrate inhibition Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00056
-
hygromycin B
-
Streptomyces hygroscopicus
0.0364
-
ATP
-
Streptomyces hygroscopicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Streptomyces hygroscopicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement, optimal activity at 0.1 mM Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
1 * 41000, SDS-PAGE Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + hygromycin B Streptomyces hygroscopicus responsible for resistance against hygromycin B, an aminocyclitol antibiotic ADP + 7''-O-phosphohygromycin B
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces hygroscopicus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
-
Streptomyces hygroscopicus

Storage Stability

Storage Stability Organism
-20°C, in 50% v/v glycerol and 0.1% bovine serum albumin, stable for up to 6 months Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + hygromycin B
-
Streptomyces hygroscopicus ADP + 7''-O-phosphohygromycin B
-
?
ATP + hygromycin B responsible for resistance against hygromycin B, an aminocyclitol antibiotic Streptomyces hygroscopicus ADP + 7''-O-phosphohygromycin B
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Streptomyces hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces hygroscopicus

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8.5 pH 6.6: about 40% of maximal activity, pH 8.5: about 65% of maximal activity Streptomyces hygroscopicus