Cloned (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
L313W | variant behaves similarly to wild type protein | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fructose 6-phosphate | - |
Thermus thermophilus | |
MgADP- | addition of MgADP has little effect the specific activity of the enzyme, but diminishes the homotropic cooperativity of fructose 6-phosphate binding, reducing the Hill numbers from 1.6 to 1 | Thermus thermophilus | |
phosphoenolpyruvate | addition leads to a decease in fructose 6-phosphate binding affinity and in heterotropically induced homotropic cooperativity in fructose 6-phosphate binding, the Hill numbers increase from 1.6 to above 2.5 without decrease in specific activity | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | allosteric regulation. At saturating fructose 6-phoshate (5 mM), the Hill number for MgATP binding is 0.8 | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P21777 | - |
- |
Thermus thermophilus DSM 579 | P21777 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41 | - |
pH 8.0, 25°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Thermus thermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Thermus thermophilus DSM 579 | ADP + D-fructose 1,6-bisphosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
MgADP- | pH 8.0, 25°C | Thermus thermophilus | |
0.0016 | - |
phosphoenolpyruvate | pH 8.0, 25°C | Thermus thermophilus | |
0.027 | - |
fructose 6-phosphate | pH 8.0, 25°C | Thermus thermophilus |