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Literature summary for 2.7.1.11 extracted from
- Structure of the apo form of Bacillus stearothermophilus phosphofructokinase (2012), Biochemistry, 51, 769-775.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli RL257 cells | Geobacillus stearothermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 0.2 M calcium acetate hydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, and 18% (w/v) polyethylene glycol 8000, at 16°C | Geobacillus stearothermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | contains Ca2+ ions | Geobacillus stearothermophilus |  |
| Mg2+ | required | Geobacillus stearothermophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | P00512 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Mimetic Blue 1 column chromatography and Mono Q column chromatography | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | x-ray crystallography | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFK | - |
Geobacillus stearothermophilus |
| phosphofructokinase | - |
Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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