Literature summary for 2.7.1.11 extracted from

Keillor, J.W.; Lherbet, C.; Castonguay, R.; Lapierre, D.; Martinez-Oyanedel, J.; Fothergill-Gilmore, L.A.; Walkinshaw, M.D.
Expression, purification, crystallization and preliminary crystallographic analysis of Trypanosoma brucei phosphofructokinase (2003), Acta Crystallogr. Sect. D, 59, 532-534.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour-diffusion, well solution consists of 1.7-1.8 M sodium formate, 100 mM sodium acetate, pH 4.6-4.8, hanging drop contains 4-5 mg/ml protein, 20 mM TEA buffer, pH 8.0, PFK is crystallized in the absence and presence of reaction products and the active site inhibitor 2,5-anhydro-1-deoxy-1-(m-nitro-D-mannitol-6-disodiumphosphate) at 2.9-4.0 A resolution	Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55585	-	4 * 55585, recombinant PFK, MALDI-TOF mass spectrometry	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PFK, metal-affinity chromatography	Trypanosoma brucei

Subunits

Subunits	Comment	Organism
tetramer	4 * 55585, recombinant PFK, MALDI-TOF mass spectrometry	Trypanosoma brucei

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Release 2023.1 (January 2023)