Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.001 mM, 28% inhibition | Thermotoga maritima | |
diphosphate | 0.1 mM, 80% inhibition, nucleotide diphosphates, i.e. ADP, GDP or TDP, partially alleviate inhibition | Thermotoga maritima | |
Zn2+ | 0.001 mM, 28% inhibition | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
ATP | pH 7.8, 50°C | Thermotoga maritima | |
0.44 | - |
D-fructose 6-phosphate | pH 7.8, 50°C | Thermotoga maritima | |
1.36 | - |
GTP | pH 7.8, 50°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 34% of activity with Mg2+ | Thermotoga maritima | |
K+ | required for activity, maximal activity at 175 mM, 50% lower activity in the absence of K+ | Thermotoga maritima | |
Mg2+ | required for activity | Thermotoga maritima | |
Mn2+ | 90% of activity with Mg2+ | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34447 | - |
alpha4, 4 * 34447, deduced from nucleotide sequence | Thermotoga maritima |
200000 | - |
recombinant PFK, gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PFK | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Thermotoga maritima | ADP + D-fructose 1,6-bisphosphate | - |
r | |
CTP + D-fructose 6-phosphate | 13% of activity with ATP | Thermotoga maritima | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | 42% of activity with ATP | Thermotoga maritima | GDP + D-fructose 1,6-bisphosphate | - |
? | |
TTP + D-fructose 6-phosphate | 10% of activity with ATP | Thermotoga maritima | TDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | 14% of activity with ATP | Thermotoga maritima | UDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 34447, deduced from nucleotide sequence | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life grater than 5 h in phosphate buffer | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8 | fructose 6-phosphate phosphorylation | Thermotoga maritima |