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Literature summary for 2.7.1.11 extracted from
- D-fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties (1969), J. Biol. Chem., 244, 6280-6288.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | above 1 mM at 0.33 mM fructose 6-phosphate and 2 mM Mg2+, inhibition at a Mg/ATP ratio below 2; ADP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; Mg2+ partially reverses inhibition | Klebsiella aerogenes |  |
| citrate | cAMP, ADP or fructose 1,6-bisphosphate restore activity | Klebsiella aerogenes | |
| CTP | - |
Klebsiella aerogenes | |
| GTP | - |
Klebsiella aerogenes | |
| additional information | not inhibited by ADP | Klebsiella aerogenes | |
| nucleoside triphosphate | at a Mg/NTP ratio below 2 | Klebsiella aerogenes | |
| UTP | - |
Klebsiella aerogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 2/1 | Klebsiella aerogenes | |
| 0.6 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 1.4/1 | Klebsiella aerogenes | |
| 1.4 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 0.7/1 | Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Klebsiella aerogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
strains PRL-R3, wild-type, and its mutant DD31 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, ammonium sulfate, Sephadex G-200, partial purification | Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.75 | - |
- |
Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Klebsiella aerogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | approx. 80% of maximal activity at pH 7 and pH 9, approx. half-maximal activity at pH 6.7 | Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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