Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.11 extracted from

  • Gottschalk, G.; von Hugo, H.
    Phosphofructokinase from Clostridium pasteurianum (1982), Methods Enzymol., 90, 82-87.
    View publication on PubMed
Search for more literature for 2.7.1.11

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Clostridium pasteurianum
Cu2+
-
 Clostridium pasteurianum
D-fructose 1,6-bisphosphate
-
 Clostridium pasteurianum
Hg2+
-
 Clostridium pasteurianum
Ni2+
-
 Clostridium pasteurianum
SO42-
-
 Clostridium pasteurianum
Tris-HCl buffer
-
 Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 ATP pH 8.0, 25°C Clostridium pasteurianum
0.62
-
 D-fructose 6-phosphate pH 8.0, 25°C Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Clostridium pasteurianum
Fe2+ activation, less effective than Mg2+ Clostridium pasteurianum
Mg2+ required for activity Clostridium pasteurianum
Mn2+ activation Clostridium pasteurianum
Zn2+ activation Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 gel filtration Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sephadex, calcium phosphate gel, Sephadex G-100, DEAE-Sephadex, hydroxyapatite Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
299
-
-
 Clostridium pasteurianum

Storage Stability

Storage Stability Organism
-20°C, in 50 mM phosphate buffer, pH 7.5, 1 year, no loss of activity Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Clostridium pasteurianum ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 9.2
-
 Clostridium pasteurianum