Cloned (Comment) | Organism |
---|---|
gene rdgA | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a mutant defective in the gene encoding DGK, rdgA | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
R59022 | a specific pharmacological enzyme inhibitor | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | rhabdomeral membrane, the enzyme is located predominantly in the cytoplasmic subrhabdomeral cisternae, a cytoplasmic membrane system located at close distance from the microvilli. Excised membrane patches retain a functional enzyme DGK | Drosophila melanogaster | 16020 | - |
rhabdomere | rhabdomeral membrane, the enzyme is located predominantly in the cytoplasmic subrhabdomeral cisternae, a cytoplasmic membrane system located at close distance from the microvilli. Excised membrane patches retain a functional enzyme DGK | Drosophila melanogaster | 16028 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | Drosophila melanogaster | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-diacyl-sn-glycerol | Drosophila melanogaster Oregon-R | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q09103 | gene rdgA | - |
Drosophila melanogaster Oregon-R | Q09103 | gene rdgA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | - |
Drosophila melanogaster | - |
photoreceptor | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | - |
Drosophila melanogaster | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-diacyl-sn-glycerol | - |
Drosophila melanogaster Oregon-R | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAG-kinase | - |
Drosophila melanogaster |
DGK | - |
Drosophila melanogaster |
eye-specific diacylglycerol kinase | UniProt | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
physiological function | light-dependent channel TRP in the light-sensitive microvilli of the photoreceptor's rhabdomere is opened by diacylglycerol and silenced by ATP, suggesting diacylglycerol kinase involvement. The ATP effect is abolished by inhibiting enzyme diacylglycerol kinase DGK and in the rdgA mutant, lacking functional DGK. Diacylglycerol activates TRP even in the presence of a DAG-lipase inhibitor, inconsistent with a requirement of polyunsaturated fatty acids in opening TRP. Diacylglycerol is the endogenous TRP agonist. ATP lowers the channel activity, the ATP effect is completely abolished by inhibition of the enzyme | Drosophila melanogaster |