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Literature summary for 2.7.1.107 extracted from

  • Decaffmeyer, M.; Shulga, Y.V.; Dicu, A.O.; Thomas, A.; Truant, R.; Topham, M.K.; Brasseur, R.; Epand, R.M.
    Determination of the topology of the hydrophobic segment of mammalian diacylglycerol kinase epsilon in a cell membrane and its relationship to predictions from modeling (2008), J. Mol. Biol., 383, 797-809.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cell, with FLAG-tag Homo sapiens

Protein Variants

Protein Variants Comment Organism
P32A redcution of both Km and kcat value, while maintianing the ratio kcat/Km constant. Specificity of mutant for substrates with polyunsaturated acyl chains is retained. Mutant has a higher affinity for membranes Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value for wild-type is 0.59 mol%, for mutant P32A 0.13 mol%, using substrate 1-stearoyl-2-oleoyl-sn-glycerol Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane segment from amino acid residues 18 to 42 forms a bent helix that enters and leaves the same side of the membrane Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P52429
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-stearoyl-2-oleoyl-sn-glycerol
-
Homo sapiens ADP + 1-stearoyl-2-oleoyl-sn-glycerol 3-phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
1-stearoyl-2-oleoyl-sn-glycerol mutant P32A Homo sapiens
2.35
-
1-stearoyl-2-oleoyl-sn-glycerol wild-type Homo sapiens