Cloned (Comment) | Organism |
---|---|
expression in COS-7 cell, with FLAG-tag | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
P32A | redcution of both Km and kcat value, while maintianing the ratio kcat/Km constant. Specificity of mutant for substrates with polyunsaturated acyl chains is retained. Mutant has a higher affinity for membranes | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for wild-type is 0.59 mol%, for mutant P32A 0.13 mol%, using substrate 1-stearoyl-2-oleoyl-sn-glycerol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | segment from amino acid residues 18 to 42 forms a bent helix that enters and leaves the same side of the membrane | Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P52429 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-stearoyl-2-oleoyl-sn-glycerol | - |
Homo sapiens | ADP + 1-stearoyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
1-stearoyl-2-oleoyl-sn-glycerol | mutant P32A | Homo sapiens | |
2.35 | - |
1-stearoyl-2-oleoyl-sn-glycerol | wild-type | Homo sapiens |