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Literature summary for 2.7.1.107 extracted from

  • Adachi, N.; Oyasu, M.; Taniguchi, T.; Yamaguchi, Y.; Takenaka, R.; Shirai, Y.; Saito, N.
    Immunocytochemical localization of a neuron-specific diacylglycerol kinase beta and gamma in the developing rat brain (2005), Mol. Brain Res., 139, 288-299.
    View publication on PubMed
Search for more literature for 2.7.1.107

Cloned(Commentary)

Cloned (Comment) Organism
GFP-tagged DGKh and DGKg co-expressed in human neuroblastoma cells SH-SY5Y, expression of GST-fusion isozyme DGKalpha and DGKgamma in Escherichia coli strain DH5alpha, expression of GST-fusion isozyme DGKbeta in Spodoptera frugiperda Sf9 cells via baculovirus infection system, expression of GST-fusion isozymes DGKalpha, DGKbeta, and DGKgamma in COS-7 cells Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isozyme DGKgamma in hippocampal pyriamidal cells and proximal dendrites Rattus norvegicus 5829
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additional information no isozyme DGKgamma in the nucleus, subcellular localization of isozymes in neurons Rattus norvegicus
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plasma membrane isozyme DGKbeta predominantly, in hippocampal pyriamidal neurons including perikarya and the peripheral dendrites, dendrit cell body Rattus norvegicus 5886
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Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, isozymes DGKalpha, DGKbeta, and DGKgamma Rattus norvegicus
Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacylglycerol Rattus norvegicus termination of diacylglycerol signaling, isozymes DGKalpha, DGKbeta, and DGKgamma play a pivotal role in development and metabolism of brain ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 neuron-specific isozymes DGKbeta and DGKgamma
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Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion isozymes DGKalpha, DGKgamma, and DGKbeta from Escherichia coli, and Sf9 cells, respectively, and from COS-7 cells, by glutathione affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain post-natal, developing, neuron-specific isozymes DGKbeta and DGKgamma, and isozyme DGKalpha, isozymes alpha and gamma appear at birth and then decline, while isozyme beta appears about 14 days after birth and reaches a maximum at day 28, overview Rattus norvegicus
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hippocampus pyramidal cells, isozymes DGKbeta and DGKgamma Rattus norvegicus
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medial geniculate nucleus isozyme DGKgamma, day 3-14 after birth Rattus norvegicus
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neuron neuron-specific isozymes DGKbeta and DGKgamma having a spatio-temporally different function in the neuron Rattus norvegicus
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Purkinje cell delay of isozyme DGKgamma Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacylglycerol
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 Rattus norvegicus ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
ATP + 1,2-diacylglycerol termination of diacylglycerol signaling, isozymes DGKalpha, DGKbeta, and DGKgamma play a pivotal role in development and metabolism of brain Rattus norvegicus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 88000-90000, isozymes DGKalpha, DGKbeta, and DGKgamma, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
DGK
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus