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Literature summary for 2.7.1.107 extracted from
- Analysis of a novel diacylglycerol kinase from Dictyostelium discoideum: DGKA (2005), Biochemistry, 44, 10199-10207.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidic acid | highly stimulating | Dictyostelium discoideum |  |
| phosphatidylinositol 4,5-bisphosphate | highly stimulating | Dictyostelium discoideum | |
| phosphatidylserine | strong activation | Dictyostelium discoideum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, follow a Michaelis-Menten mechanism in case of medium-chain diacylglycerols, and are strongly dependent on detergent concentration and sort used in the micelles in the assay in case of longer-chain substrates showing sigmoidal kinetics, overview | Dictyostelium discoideum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1,2-diacylglycerol | Dictyostelium discoideum | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-diacylglycerol | Dictyostelium discoideum HL5 | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | - |
- |
- |
| Dictyostelium discoideum HL5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme, by ultracentrifugation, gel filtration, and antibody affinity chromatography | Dictyostelium discoideum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate | catalytic and regulatory mechanisms | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1,2-diacylglycerol | - |
Dictyostelium discoideum | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-diacylglycerol | - |
Dictyostelium discoideum HL5 | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-dihexanoyl-sn-glycerol | - |
Dictyostelium discoideum | ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-dihexanoyl-sn-glycerol | - |
Dictyostelium discoideum HL5 | ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-dioctanoyl-sn-glycerol | preferred substrate | Dictyostelium discoideum | ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-dioctanoyl-sn-glycerol | preferred substrate | Dictyostelium discoideum HL5 | ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-dioleoyl-sn-glycerol | - |
Dictyostelium discoideum | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1,2-dioleoyl-sn-glycerol | - |
Dictyostelium discoideum HL5 | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DGKA | - |
Dictyostelium discoideum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Dictyostelium discoideum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Dictyostelium discoideum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Dictyostelium discoideum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Dictyostelium discoideum | |
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