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Literature summary for 2.7.1.105 extracted from
- ATP-dependent activation of a new form of spinach leaf 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (1987), Arch. Biochem. Biophys., 258, 58-64.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | one enzyme form is activated dramatically as result of modification by MgATP during preincubation | Spinacia oleracea |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Spinacia oleracea |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | Spinacia oleracea | enzyme plays an important role in the regulation of hepatic carbohydrate metabolism | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 distinct molecular forms | Spinacia oleracea |
| partial | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, partially purified, less than 10% loss of activity within 1 month | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | - |
Spinacia oleracea | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
| ATP + beta-D-fructose 6-phosphate | enzyme plays an important role in the regulation of hepatic carbohydrate metabolism | Spinacia oleracea | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.9 | - |
Spinacia oleracea |
| 7.6 | - |
- |
Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.2 | about half-maximal activity at pH 7.0 and 8.2, peak II enzyme | Spinacia oleracea |
| 7.1 | 8.3 | about half-maximal activity at pH 7.1 and 8.3, peak 1 enzyme | Spinacia oleracea |
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Release 2023.1 (January 2023)
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