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Literature summary for 2.7.1.105 extracted from

  • Strohecker, A.M.; Joshi, S.; Possemato, R.; Abraham, R.T.; Sabatini, D.M.; White, E.
    Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel autophagy regulator by high content shRNA screening (2015), Oncogene, 34, 5662-5676.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information knockdown of PFKFB4 in prostate cancer cells by specific shRNA targeting Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Homo sapiens
-
ADP + beta-D-fructose 2,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16877 gene/isozyme Pfkfb4
-

Source Tissue

Source Tissue Comment Organism Textmining
PC-3 cell increased expression of PFKFB4 in multiple solid tumor types including breast, colon, and prostate Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate
-
?

Synonyms

Synonyms Comment Organism
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
Homo sapiens
PFKFB4
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
malfunction knockdown of PFKFB4 in prostate cancer cells increases p62 and reactive oxygen species, but surprisingly increases autophagic flux. Addition of the reactive oxygen species scavenger N-acetyl cysteine prevents p62 accumulation in PFKFB4-depleted cells. PFKFB4 depletion acts upstream of ATG7 consistent with increased oxidative stress that induces autophagy and p62 upregulation Homo sapiens
metabolism upregulation of p62 and autophagy is a response to oxidative stress caused by PFKFB4. PFKFB4 is an autophagy regulator Homo sapiens
physiological function the putative autophagy stimulator, isozyme PFKFB4, drives flux through pentose phosphate pathway. PFKFB4 suppresses oxidative stress and p62 accumulation, without which autophagy is stimulated likely as a reactive oxygen species detoxification response. Genes whose loss enhanced p62 elimination are putative negative regulators of autophagy and the bi-functional enzyme PFKFB4 highly inhibits p62 elimination. PFKFB4 is an autophagy regulator. PFKFB4 suppresses autophagy and p62 accumulation by mitigating reactive oxygen species Homo sapiens