Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA silencing of endogenous PFKFB3 in HeLa cells by siRNA | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one | the PFKFB3 inhibitor, 3PO, increases p27 protein in Lewis lung carcinoma cells in vitro and in vivo | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Homo sapiens | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16875 | gene PFKFB3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-116 cell | PFKFB3 is overexpressed in human cancers | Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
HeLa cell | PFKFB3 is overexpressed in human cancers | Homo sapiens | - |
Lewis lung carcinoma cell | - |
Homo sapiens | - |
LLC cell | PFKFB3 is overexpressed in human cancers | Homo sapiens | - |
additional information | PFKFB3 is overexpressed in human cancers | Homo sapiens | - |
PANC-1 cell | PFKFB3 is overexpressed in human cancers | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Homo sapiens | ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo-2-kinase | - |
Homo sapiens |
PFKFB3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | siRNA silencing of endogenous PFKFB3 inhibits Cdk1 activity, which in turn stabilizes p27 protein levels causing cell cycle arrest at G1/S and increased apoptosis in HeLa cells. PFKFB3 inhibition completely suppresses cell proliferation and results in increased early and late apoptotic cells. PFKFB3 inhibition results in increased nuclear and cytoplasmic p27 protein but has no effect on p57 or p21. Blockade of cell cycle progression and stimulation of apoptosis by PFKFB3 inhibition is mediated by p27 | Homo sapiens |
metabolism | the family of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) comprises well established regulators of glucose metabolism via their synthesis of fructose-2,6 bisphosphate, a potent allosteric activator of 6-phosphofructo-1-kinase. But PFKFB3 and fructose-2,6 bisphosphate function not only as regulators of Pfk-1 but also of Cdk1 activity, and therefore serve to couple glucose metabolism with cell proliferation and survival in transformed cells | Homo sapiens |
physiological function | PFKFB3 is overexpressed in human cancers, regulated by HIF-1alpha, Akt and PTEN, and required for the survival and growth of multiple cancer types. Role of PFKFB3 in regulating Cdk1- and p27-mediated G1/S blockade and apoptosis | Homo sapiens |