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Literature summary for 2.7.1.105 extracted from

  • Novellasdemunt, L.; Tato, I.; Navarro-Sabate, A.; Ruiz-Meana, M.; Mendez-Lucas, A.; Perales, J.C.; Garcia-Dorado, D.; Ventura, F.; Bartrons, R.; Rosa, J.L.
    Akt-dependent activation of the heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) isoenzyme by amino acids (2013), J. Biol. Chem., 288, 10640-10651.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information amino acids increase beta-D-fructose 2,6-bisphosphate concentration in HeLa and in MCF-7 human cells, molecular mechanisms involved differ from those observed with insulin. Only essential amino acids without glutamine are required to activate the PI3K/mTORC2 and p38/MAPKAPK-2 pathways and to increase the beta-D-fructose 2,6-bisphosphate concentration. The observed effects in signaling and metabolism are produced by a mixture of essential amino acids Homo sapiens
additional information amino acids increase beta-D-fructose 2,6-bisphosphate concentration in primary cardiomyocytes, molecular mechanisms involved differ from those observed with insulin. Only essential amino acids without glutamine are required to activate the PI3K/mTORC2 and p38/MAPKAPK-2 pathways and to increase the beta-D-fructose 2,6-bisphosphate concentration. The observed effects in signaling and metabolism are produced by a mixture of essential amino acids Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
gene PFKFB2 Homo sapiens
gene PFKFB2 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Homo sapiens
-
ADP + beta-D-fructose 2,6-bisphosphate
-
?
ATP + beta-D-fructose 6-phosphate Rattus norvegicus
-
ADP + beta-D-fructose 2,6-bisphosphate
-
?
ATP + beta-D-fructose 6-phosphate Rattus norvegicus Sprague-Dawley
-
ADP + beta-D-fructose 2,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60825 gene/isozyme PFKFB2
-
Rattus norvegicus Q9JJH5 gene/isozyme PFKFB2
-
Rattus norvegicus Sprague-Dawley Q9JJH5 gene/isozyme PFKFB2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein specific phosphorylation of heart 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoenzyme PFKFB2 at Ser483. This activation is mediated by the phosphatidylinositol-4,5-bisphosphate 3-kinase and p38 signaling pathways. Amino acid-activated Akt phosphorylates PFKFB2 at Ser483. Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Homo sapiens
phosphoprotein specific phosphorylation of heart 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoenzyme PFKFB2 at Ser483. This activation is mediated by the phosphatidylinositol-4,5-bisphosphate 3-kinase and p38 signaling pathways. Amino acid-activated Akt phosphorylates PFKFB2 at Ser483. Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
cardiomyocyte primary Rattus norvegicus
-
heart heart isozyme PFKFB2 Homo sapiens
-
heart heart isozyme PFKFB2 Rattus norvegicus
-
HeLa cell
-
Homo sapiens
-
MCF-7 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate
-
?
ATP + beta-D-fructose 6-phosphate
-
Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
?
ATP + beta-D-fructose 6-phosphate
-
Rattus norvegicus Sprague-Dawley ADP + beta-D-fructose 2,6-bisphosphate
-
?

Synonyms

Synonyms Comment Organism
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
Homo sapiens
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
Rattus norvegicus
PFKFB2
-
Homo sapiens
PFKFB2
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 assay at Homo sapiens
7.5 8 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
ATP
-
Rattus norvegicus

General Information

General Information Comment Organism
malfunction Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Homo sapiens
malfunction Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Rattus norvegicus