Literature summary for 2.7.1.105 extracted from

Baez, M.; Babul, J.
Reversible unfolding of dimeric phosphofructokinase-2 from Escherichia coli reveals a dominant role of inter-subunit contacts for stability (2009), FEBS Lett., 583, 2054-2060.

Search for more literature for 2.7.1.105

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	unfolded monomer, determined by gel filtration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Cibacron blue-Sepharose and AMP-agarose chromatography	Escherichia coli

Renatured (Commentary)

Renatured (Comment)	Organism
for refolding experiments, the enzyme is first exposed to 3 M GdnHCl for 5 h at 20°C, under this condition Pfk-2 is completely unfolded, the refolding curve is obtained diluting the unfolded enzyme to several guanidine hydrochloride concentrations in the 50 mM Tris, pH 7.8, 5 mM MgCl2 and 10 mM dithiotreitol, under these conditions equilibrium is achieved after 5 h of incubation	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity recovery after refolding is 90% in the full range of GdnHCl concentrations of 0.1-5.5 M guanidine hydrochloride	Escherichia coli

Subunits

Subunits	Comment	Organism
homodimer	determined by circular dichroismand gel filtration, subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunits beta-sheet, Pfk-2 undergoes a cooperative unfolding/dissociation process with the accumulation of an expanded and unstructured monomeric intermediate with a marginal stability and a large solvent accessibility with respect to the native dimer	Escherichia coli

Synonyms

Synonyms	Comment	Organism
Pfk-2	-	Escherichia coli
phosphofructokinase-2	-	Escherichia coli

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Release 2023.1 (January 2023)