Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyrene maleimide | incorporation of 2 mol per mol of enzyme subunit, modifiying Cys-238 and Cys-295, leads to rapid inactivation, MgATP2- protects Cys295, modification of Cys238 does not abolish activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of native and pyrene maleimide modified enzyme | Escherichia coli | |
0.042 | - |
MgATP2- | native enzyme | Escherichia coli | |
0.044 | - |
MgATP2- | C238-pyrene maleimide modified enzyme | Escherichia coli | |
0.058 | - |
beta-D-fructose 6-phosphate | native enzyme | Escherichia coli | |
0.063 | - |
beta-D-fructose 6-phosphate | C238-pyrene maleimide modified enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | as MgATP2- | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Escherichia coli | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | ordered bi bi mechanism, structural and functional roles of Cys-238 and Cys-295 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Escherichia coli | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
MgATP2- + beta-D-fructose 6-phosphate | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type enzyme in presence of MgATP2-, and C238-pyrene maleimide modified enzyme in presence of beta-D-fructose 6-phosphate and ATP4- | Escherichia coli |
monomer | C238-pyrene maleimide modified enzyme in absence of MgATP2- | Escherichia coli |
tetramer | wild-type enzyme in absence of ligands, and C238-pyrene maleimide modified enzyme in presence of MgATP2- | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Pfk-2 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9240 | - |
MgATP2- | native enzyme | Escherichia coli | |
9240 | - |
beta-D-fructose 6-phosphate | native enzyme | Escherichia coli | |
9320 | - |
MgATP2- | C238-pyrene maleimide modified enzyme | Escherichia coli | |
9320 | - |
beta-D-fructose 6-phosphate | C238-pyrene maleimide modified enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | as MgATP2- | Escherichia coli |