Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | - |
Sparus aurata | |
additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme, not skeletal muscle enzyme | Sparus aurata | |
phosphoenolpyruvate | - |
Sparus aurata | |
sn-glycerol 3-phosphate | most potent inhibitor of phosphorylated liver enzyme, phosphorylation enhances sensitivity; skeletal muscle enzyme is not sensitive to inhibition | Sparus aurata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
beta-D-fructose 2,6-bisphosphate | pH 7.4, 30°C, in presence of phosphate | Sparus aurata | |
0.223 | - |
ATP | pH 7.4, 30°C, presence of phosphate | Sparus aurata | |
0.94 | - |
beta-D-fructose 6-phosphate | pH 7.4, 30°C, presence of phosphate | Sparus aurata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sparus aurata | - |
teleost fish | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme, not skeletal muscle enzyme | Sparus aurata |
Purification (Comment) | Organism |
---|---|
skeletal muscle | Sparus aurata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Sparus aurata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sparus aurata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Sparus aurata | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Sparus aurata | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sparus aurata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
citrate | pH 7.4, 30°C | Sparus aurata | |
0.23 | - |
phosphoenolpyruvate | pH 7.4, 30°C | Sparus aurata |