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Literature summary for 2.7.1.105 extracted from

  • García de Frutos, P.; Baanante, I.V.
    6-Phosphofructo 2-kinase/fructose 2,6-bisphosphatase in liver of the teleost Sparus aurata (1994), Arch. Biochem. Biophys., 308, 461-468.
    View publication on PubMed
Search for more literature for 2.7.1.105

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Sparus aurata
citrate phosphorylation enhances sensitivity Sparus aurata
additional information no inhibition by lactate, glyceraldehyde 3-phosphate, beta-D-fructose 1,6-bisphosphate Sparus aurata
phosphoenolpyruvate mixed-type inhibitory effect, phosphorylation enhances sensitivity Sparus aurata
sn-glycerol 3-phosphate most potent inhibitor of phosphorylated liver enzyme, phosphorylation enhances sensitivity Sparus aurata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data of native and phosphorylated enzyme Sparus aurata
0.018
-
 ATP pH 7.5, 30°C, in presence of phosphate Sparus aurata
0.156
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C, with ATP, in presence of phosphate Sparus aurata
0.226
-
 ATP pH 7.5, 30°C, absence of phosphate Sparus aurata
1.4
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C, with ATP, absence of phosphate Sparus aurata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 2 * 54000, SDS-PAGE Sparus aurata
98000
-
 gel filtration Sparus aurata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Sparus aurata
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sparus aurata
-
 teleost fish
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Sparus aurata

Purification (Commentary)

Purification (Comment) Organism
liver Sparus aurata

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Sparus aurata
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.068
-
-
 Sparus aurata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Sparus aurata ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
ATP + beta-D-fructose 6-phosphate
-
 Sparus aurata ADP + beta-D-fructose 2,6-bisphosphate via phosphorylenzyme intermediate r
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Sparus aurata ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Sparus aurata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sparus aurata

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Sparus aurata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.17
-
 phosphoenolpyruvate noncompetitive to ATP, pH 7.5, 30°C Sparus aurata
0.53
-
 phosphoenolpyruvate noncompetitive to fructose 6-phosphate, pH 7.5, 30°C Sparus aurata
0.55
-
 citrate pH 7.5, 30°C Sparus aurata