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Literature summary for 2.7.1.105 extracted from

  • Pilkis, S.J.; Regen, D.M.; Stewart, H.B.; Pilkis, J.; Pate, T.M.; El-Maghrabi, M.R.
    Evidence for two catalytic sites on 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase. Dynamics of substrate exchange and phosphoryl enzyme formation (1984), J. Biol. Chem., 259, 949-958.
    View publication on PubMed
Search for more literature for 2.7.1.105

Inhibitors

Inhibitors Comment Organism Structure
ADP product inhibition Rattus norvegicus
AMP not Rattus norvegicus
beta-D-fructose 2,6-bisphosphate product inhibition Rattus norvegicus
citrate not Rattus norvegicus
sn-glycerol 3-phosphate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus
0.05
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C, with ATP, in presence of phosphate Rattus norvegicus
0.1
-
 ATP pH 7.5, 30°C Rattus norvegicus
0.5
-
 MgATP2- pH 7.5, 30°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgATP2 Rattus norvegicus
phosphate activation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate mechanism Rattus norvegicus
a
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate via phosphorylenzyme intermediate r
MgATP2- + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ?
-
 ?
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?
additional information isotope exchange reaction between ATP and ADP in the absence of fructose 6-phosphate Rattus norvegicus ?
-
 ?
additional information with discrete catalytic sites Rattus norvegicus ?
-
 ?
additional information isotope exchange between fructose 6-phosphate and fructose 2,6-bisphosphate in the absence of adenine nucleotides Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus