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Literature summary for 2.7.1.1 extracted from
- Glycerol 3-phosphate alters Trypanosoma brucei hexokinase activity in response to environmental change (2011), J. Biol. Chem., 286, 33150-33157.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glycerol 3-phosphate | influences enzyme activity at pH 6.5 by preventing substrate and product inhibition by ATP and ADP, respectively | Trypanosoma brucei |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His-tagged fusion protein in Escherichia coli | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Trypanosoma brucei | |
| quercetin | quercetin inhibition, but not binding, is relieved by glycerol 3-phosphate at pH 6.5 | Trypanosoma brucei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
ATP | pH 6.5, temperature not specified in the publication | Trypanosoma brucei | |
| 0.28 | - |
ATP | pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using affinity and ion-exchange chromatography | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Trypanosoma brucei | ADP + D-glucose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hexokinase | - |
Trypanosoma brucei |
| TbHK1 | - |
Trypanosoma brucei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
enzyme shows no activity | Trypanosoma brucei |
| 7 | - |
50% activity | Trypanosoma brucei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
hexokinase activity is pH-sensitive, but glycerol 3-phosphate prevents inactivation even maintaining detectable enzyme activity at pH 4.0 | Trypanosoma brucei |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 4.2 | - |
pH 6.5, temperature not specified in the publication | Trypanosoma brucei | ADP | |
| 10 | - |
pH 7.4, temperature not specified in the publication | Trypanosoma brucei | ADP | |
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