Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.1 extracted from

  • Zhang, J.; Li, C.; Shi, T.; Chen, K.; Shen, X.; Jiang, H.
    Lys169 of human glucokinase is a determinant for glucose phosphorylation: implication for the atomic mechanism of glucokinase catalysis (2009), PLoS ONE, 4, e6304.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine glucokinase is a potential therapeutic target for treating maturity-onset diabetes of the young and persistent hyperinsulinemic hypoglycemia of infancy Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain M15 Homo sapiens

Protein Variants

Protein Variants Comment Organism
K169A inactive, Lys169 enhances the binding of glucokinase with both ATP and glucose by serving as a bridge between ATP and glucose, Lys169 directly participates in the glucose phosphorylation as a general acid catalyst Homo sapiens
K169N naturally occurring mutation in the glucokinase gene associated with familial mild fasting hyperglycemia Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
ATP wild type enzyme, in 25 mM HEPES (pH 7.1), at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P19367
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
Homo sapiens ADP + D-glucose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
glucokinase
-
Homo sapiens
LGK2
-
Homo sapiens
liver glucokinase isoform 2
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens