Cloned (Comment) | Organism |
---|---|
wild type, mutant V62M,and G72R recombinant full-length human beta-cell glucokinases are expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G72R | mutant enzymes shows reduced rates of turnover (20% relative activity at 30°C, 10% relative activity at 37°C) and reduced glucose affinity relative to wild type, the mutant loses the stabilizing protein interactions with glucokinase regulatory protein, which may contribute to lower activity in vivo, the mutation associates with hyperglycemia in humans | Homo sapiens |
V62M | mutant enzymes shows reduced rates of turnover (70% relative activity at 30°C, 40% relative activity at 37°C) and reduced glucose affinity relative to wild type, the mutant loses the stabilizing protein interactions with glucokinase regulatory protein, which may contribute to lower activity in vivo, the mutation associates with hyperglycemia in humans | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | wild type enzyme, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
0.77 | - |
ATP | wild type enzyme, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
0.82 | - |
ATP | mutant enzyme V62M, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
0.85 | - |
ATP | mutant enzyme V62M, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
1.1 | - |
ATP | mutant enzyme G72R, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
1.1 | - |
ATP | mutant enzyme G72R, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35557 | - |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography, ammonium sulfate precipitation, Q-Sepharose column chromatography, glutathione-Sepharose 4B column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MODY2 glucokinase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | 52 | half-life of 5-7 min at 50°C, the transition temperatures for the wild type enzyme are 48.4°C and 51.6°C in the absence and presence of saturating glucose (100 mM), respectively | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
D-glucose | mutant enzyme G72R, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
34 | - |
D-glucose | mutant enzyme G72R, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
54 | - |
D-glucose | mutant enzyme V62M, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
61 | - |
D-glucose | mutant enzyme V62M, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
74 | - |
D-glucose | wild type enzyme, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens | |
101 | - |
D-glucose | wild type enzyme, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |