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Literature summary for 2.7.1.1 extracted from
- Bovine hexokinase type I: full-length cDNA sequence and characterisation of the recombinant enzyme (2005), Mol. Cell. Biochem., 268, 9-18.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| type I enzyme, DNA and amino acid sequence determination and analysis, functional expression of soluble full length enzyme and of the soluble C-terminal domain in Escherichia coli strain BL21(DE3) | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Glucose 1,6-bisphosphate | competitive versus MgATP2-, low concentration of phosphate counteract | Bos taurus |  |
| D-glucose 6-phosphate | competitive versus MgATP2- | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.047 | - |
D-glucose | pH 7.2, 37°C, recombinant catalytic C-terminal domain | Bos taurus | |
| 0.072 | - |
D-glucose | pH 7.2, 37°C, recombinant full length enzyme | Bos taurus | |
| 0.67 | - |
ATP | pH 7.2, 37°C, recombinant full length enzyme | Bos taurus | |
| 0.67 | - |
ATP | ATP in form of MgATP2- | Bos taurus | |
| 0.98 | - |
ATP | pH 7.2, 37°C, recombinant catalytic C-terminal domain | Bos taurus | |
| 0.98 | - |
ATP | ATP in form of MgATP2- | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | MgATP2- | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE | Bos taurus |
| 111000 | - |
x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Bos taurus | involved in glucose catabolism | ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | Q5W5U3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant full length enzyme and C-terminal domain from Escherichia coli strain BL21(DE3) by ion exchange, hydrophobic interaction, anddye ligand affinity chromatography, and gel filtration | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.44 | - |
cell extract of recombinant Escherichia coli expressing the full length enzyme | Bos taurus |
| 3.5 | - |
cell extract of recombinant Escherichia coli expressing the catalytic C-terminal domain of the enzyme | Bos taurus |
| 94 | - |
purified recombinant full length enzyme | Bos taurus |
| 141 | - |
purified recombinant catalytic C-terminal domain of the enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-2-fluoro-D-glucose | - |
Bos taurus | ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Bos taurus | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | involved in glucose catabolism | Bos taurus | ADP + D-glucose 6-phosphate | - |
? | |
| additional information | the active site is located in the C-terminal domain | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE | Bos taurus |
| More | the active site is located in the C-terminal domain | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hexokinase type I | - |
Bos taurus |
| HK1 | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | MgATP2- | Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
D-Glucose 1,6-bisphosphate | pH 7.2, 37°C, recombinant catalytic C-terminal domain, versus MgATP2- | Bos taurus | |
| 0.036 | - |
D-Glucose 1,6-bisphosphate | pH 7.2, 37°C, recombinant full length enzyme, versus MgATP2- | Bos taurus | |
| 0.045 | - |
D-glucose 6-phosphate | pH 7.2, 37°C, recombinant full length enzyme | Bos taurus | |
| 0.066 | - |
D-glucose 6-phosphate | pH 7.2, 37°C, recombinant catalytic C-terminal domain | Bos taurus | |
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