Cloned (Comment) | Organism |
---|---|
cloning and overexpression of a full-length HK I, a truncate form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 1,6-diphosphate | strong inhibition of a recombinant full-length HK I, a truncated form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain, strongly pH-dependent, inhibition is reversed by phosphate, except that of the C-terminal half | Homo sapiens | |
D-glucose 6-phosphate | competitive to MgATP2-; strong inhibition of a recombinant full-length HK I, a truncated form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain, at low concentrations, 0-3 mM, inhibition is reversed by phosphate, except that of the C-terminal half | Homo sapiens | |
phosphate | at high concentrations, 10-50 mM, inhibition of recombinant full-length HK I, a truncated form lacking the first 11 amino acids named HK-11aa, and of the 50 kDa C-terminal half containing the catalytic domain, competitive versus MgATP2- | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | 0.065 | D-glucose | recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 | Homo sapiens | |
1.1 | 1.4 | ATP | MgATP2-, recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant hexokinase I | - |
Purification (Comment) | Organism |
---|---|
recombinant full-length HK I, truncate form lacking the first 11 amino acids named HK-11aa, 50 kDa C-terminal half containing the catalytic domain | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the first 11 N-terminal amino acids influence the specific activity of HK | Homo sapiens |
2 | - |
unpurified recombinant HK I and 50 kDa C-terminal half of HK I, at 22°C | Homo sapiens |
3.8 | - |
unpurified recombinant HK-11aa, a truncated HK I form lacking the first 11 amino acids, at 22°C | Homo sapiens |
144 | - |
purified recombinant HK I, at 22°C | Homo sapiens |
180 | - |
purified recombinant HK-11aa, a truncated HK I form lacking the first 11 amino acids, at 22°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | MgATP2- | Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
additional information | the 50 kDa C-terminal half of HK I contains the catalytic domain, the N-terminal half is catalytically inactive | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | 0.037 | D-glucose 6-phosphate | recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 | Homo sapiens | |
0.05 | 0.059 | D-glucose 1,6-diphosphate | recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 | Homo sapiens | |
33 | - |
phosphate | recombinant HK I, a truncate HK I form lacking the first 11 amino acids named HK-11aa, and the 50 kDa C-terminal half of HK I, at 37°C, pH 7.2 | Homo sapiens |