Cloned (Comment) | Organism |
---|---|
full-length cDNA encoding brain hexokinase I is cloned, expression of wild-type and nondimerizing mutant hexokinase I in Escherichia coli ZSC13 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structures of hexokinase I dimers | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E280A | hexokinase I mutation eliminates hydrogen bonds between monomers of crystallographic dimers, triple mutant E280A/R283A/G284Y: nonaggregating mutant of recombinant hexokinase I, engineered by directed mutation to block dimerization, exhibits wild-type activity | Homo sapiens |
G284Y | hexokinase I mutation introduces steric hindrance, triple mutant E280A/R283A/G284Y: nonaggregating mutant of recombinant hexokinase I, engineered by directed mutation to block dimerization, exhibits wild-type activity | Homo sapiens |
R283A | hexokinase I mutation eliminates hydrogen bonds between monomers of crystallographic dimers, triple mutant E280A/R283A/G284Y: nonaggregating mutant of recombinant hexokinase I, engineered by directed mutation to block dimerization, exhibits wild-type activity | Homo sapiens |
General Stability | Organism |
---|---|
phosphate stabilizes the wild-type monomer of hexokinase I relative to the dimer, D-glucose 6-phosphate stabilizes the dimer relative to the monomer | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-Anhydro-D-glucitol 6-phosphate | wild-type and nonaggregating interface mutant hexokinase I, inhibition is relieved by phosphate | Homo sapiens | |
D-glucose 6-phosphate | potent product inhibition, allosterically relieved by phosphate, wild-type hexokinase I and nondimerizing mutant | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | 0.061 | D-glucose | wild-type and nonaggregating interface mutant hexokinase I, pH 7.8 | Homo sapiens | |
0.66 | 1.2 | ATP | wild-type and nonaggregating interface mutant hexokinase I, pH 7.8 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Homo sapiens | hexokinase I governs the rate-limiting step of glycolysis in brain | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P19367 | nonaggregating mutant of recombinant hexokinase I | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and nondimerizing mutant hexokinase I, expressed in Escherichia coli ZSC13 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | hexokinase I | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60 | 61 | wild-type and nonaggregating interface mutant hexokinase I, pH 7.8 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | hexokinase I governs the rate-limiting step of glycolysis in brain | Homo sapiens | D-glucose 6-phosphate + ADP | - |
? | |
additional information | structure model of hexokinase I | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type hexokinase I is a dimer in the presence of glucose 6-phosphate and down to protein concentrations as low as 1 mg/ml | Homo sapiens |
monomer | wild-type hexokinase I is a monomer in the presence of glucose and phosphate at protein concentrations up to 10 mg/ml | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 6000/min, wild-type and nonaggregating interface mutant hexokinase I | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
1,5-Anhydro-D-glucitol 6-phosphate | nonaggregating interface mutant hexokinase I, pH 7.8 | Homo sapiens | |
23 | - |
1,5-Anhydro-D-glucitol 6-phosphate | wild-type hexokinase I, pH 7.8 | Homo sapiens |