Any feedback?
Literature summary for 2.7.1.1 extracted from
- Purification and characterization of the hexokinase from Schistosoma mansoni, expressed in Escherichia coli (1996), Protein Expr. Purif., 8, 374-380.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hexokinase cDNA is cloned, sequenced and expressed in Escherichia coli as a fusion protein including an N-terminal polyhistidine tag and enterokinase cleavage site | Schistosoma mansoni |
General Stability
| General Stability | Organism |
|---|---|
| monothioglycerol stabilizes, enzyme activity is highly dependent on maintenance of free SH-groups | Schistosoma mansoni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,5-Anhydro-D-glucitol 6-phosphate | less effective than glucose 6-phosphate, competitive versus ATP | Schistosoma mansoni |  |
| D-glucose 6-phosphate | competitive versus ATP; moderate inhibition | Schistosoma mansoni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
D-glucose | at pH 8.5 | Schistosoma mansoni | |
| 0.93 | - |
ATP | at pH 8.5 | Schistosoma mansoni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50400 | - |
x * 55000, calculated from the amino acid sequence, x * 50400, 52 kDa with His-tag at the N-terminus, SDS-PAGE | Schistosoma mansoni |
| 55000 | - |
x * 55000, calculated from the amino acid sequence, x * 50400, 52 kDa with His-tag at the N-terminus, SDS-PAGE | Schistosoma mansoni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma mansoni | - |
recombinant hexokinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant hexokinase, expressed in Escherichia coli | Schistosoma mansoni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Schistosoma mansoni |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 20 mM Hepes, 1 mM EDTA, 20 mM glucose, 10 mM monothioglycerol, pH 7.5, several months, stable | Schistosoma mansoni |
| 4°C, several days, progressive loss of activity | Schistosoma mansoni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ATP | - |
Schistosoma mansoni | ADP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, calculated from the amino acid sequence, x * 50400, 52 kDa with His-tag at the N-terminus, SDS-PAGE | Schistosoma mansoni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Schistosoma mansoni |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | variations within the range have no substantial effect on stability during purification procedure | Schistosoma mansoni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Schistosoma mansoni | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
D-glucose 6-phosphate | at pH 8.5 | Schistosoma mansoni | |
| 0.5 | - |
1,5-Anhydro-D-glucitol 6-phosphate | at pH 8.5 | Schistosoma mansoni | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Schistosoma mansoni | analytical isoelectric focusing | - |
6 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
