Cloned (Comment) | Organism |
---|---|
cDNA encoding hexokinase I is cloned and expressed in Escherichia coli BL21(DE3), amino acid sequence | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structure of hexokinase I from brain complexed with glucose and glucose 6-phosphate | Homo sapiens |
hanging drop method | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-phosphate | potent inhibition of hexokinase I is relieved by physiological levels of phosphate, mechanism | Homo sapiens | |
N-acetyl-D-glucosamine | potent inhibitor of hexokinase I | Homo sapiens | |
N-methylglucosamine | potent inhibitor of hexokinase I | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
2 * 100000 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Homo sapiens | hexokinase I is the pacemaker of glycolysis in brain tissue | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant hexokinase I, expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant hexokinase I expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | hexokinase I | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine + ATP | - |
Homo sapiens | ADP + D-glucosamine 6-phosphate | - |
? | |
D-glucose + ATP | bound glucose is present as the beta-anomer, glucose binding sites | Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | hexokinase I is the pacemaker of glycolysis in brain tissue | Homo sapiens | D-glucose 6-phosphate + ADP | - |
? | |
additional information | C-terminal catalytic and N-terminal regulatory domain, detailed structure of hexokinase I, substrate binding sites, models for regulation | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 100000 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |