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Literature summary for 2.7.1.1 extracted from

  • Renz, A.; Stitt, M.
    Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubers (1993), Planta, 190, 166-175.
No PubMed abstract available
Search for more literature for 2.7.1.1

Inhibitors

Inhibitors Comment Organism Structure
ADP isoenzymes HK1 and HK2, competitive to ATP, 0.5 mM: 4-5fold inhibition Solanum tuberosum
AMP weak Solanum tuberosum
D-glucose 6-phosphate isoenzyme HK1, not HK2, noncompetitive to glucose Solanum tuberosum
UDP weak Solanum tuberosum
UMP very weak Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 D-glucose HK3 Solanum tuberosum
0.038
-
 D-mannose HK3 Solanum tuberosum
0.041
-
 D-glucose HK1 Solanum tuberosum
0.052
-
 D-mannose HK1 Solanum tuberosum
0.09
-
 ATP HK1 Solanum tuberosum
0.13
-
 D-glucose HK2 Solanum tuberosum
0.28
-
 ATP HK2 Solanum tuberosum
0.29
-
 D-mannose HK2 Solanum tuberosum
0.56
-
 ATP HK3 Solanum tuberosum
8.7
-
 D-fructose HK3 Solanum tuberosum
11
-
 D-fructose HK1 Solanum tuberosum
22
-
 D-fructose HK2 Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+-dependent Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Solanum tuberosum glucose metabolism D-glucose 6-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
 cv. Desiree, 3 isoenzymes: HK1, HK2, HK3
-

Purification (Commentary)

Purification (Comment) Organism
partial Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber developing Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + ATP HK1: 4% of the activity with glucose, HK2: 3% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
D-fructose + ATP HK3: 5% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
D-glucose + ATP
-
 Solanum tuberosum ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP glucose metabolism Solanum tuberosum D-glucose 6-phosphate + ADP
-
 ?
D-mannose + ATP HK1: 53% of the activity with glucose, HK2: 22% of the activity with glucose, HK3: 45% of the activity with glucose Solanum tuberosum ADP + D-mannose 6-phosphate
-
 ?
GTP + D-glucose
-
 Solanum tuberosum GDP + D-glucose 6-phosphate
-
 ?
UTP + D-glucose
-
 Solanum tuberosum UDP + D-glucose 6-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 8.2 HK 1 Solanum tuberosum
7.5 9 HK 2 Solanum tuberosum
8
-
 assay at Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
6.7 9 pH 6.7: about 40% of activity maximum, pH 9: about 70% of activity maximum, isoenzyme HK1 Solanum tuberosum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Solanum tuberosum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
 ADP HK1 Solanum tuberosum
0.108
-
 ADP HK2 Solanum tuberosum
4.1
-
 D-glucose 6-phosphate HK1, at pH 7 Solanum tuberosum