General Stability | Organism |
---|---|
D-glucose or D-fructose, 5 mM, stabilizes | Oryctolagus cuniculus |
glycerol, 9-15% v/v, stabilizes | Oryctolagus cuniculus |
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-glucose | reticulocyte hexokinase Ia | Oryctolagus cuniculus | |
0.04 | - |
D-glucose | at 37°C, pH 8.1 | Oryctolagus cuniculus | |
0.1 | - |
D-mannose | hexokinase Ia | Oryctolagus cuniculus | |
0.125 | - |
D-glucose | at 37°C, pH 8.1 | Oryctolagus cuniculus | |
0.125 | - |
D-glucose | reticulocyte hexokinase Ib | Oryctolagus cuniculus | |
0.15 | - |
D-mannose | hexokinase Ib | Oryctolagus cuniculus | |
0.5 | - |
ATP | MgATP2- | Oryctolagus cuniculus | |
0.5 | - |
ATP | reticulocyte hexokinase Ia and Ib, at 37°C, pH 8.1 | Oryctolagus cuniculus | |
0.85 | - |
D-glucosamine | hexokinase Ia | Oryctolagus cuniculus | |
0.9 | - |
2-deoxy-D-glucose | hexokinase Ia | Oryctolagus cuniculus | |
1.3 | - |
D-glucosamine | hexokinase Ib | Oryctolagus cuniculus | |
2 | - |
2-deoxy-D-glucose | hexokinase Ib | Oryctolagus cuniculus | |
4 | - |
D-fructose | hexokinase Ia | Oryctolagus cuniculus | |
4.5 | - |
ITP | MgITP2-, hexokinase Ib | Oryctolagus cuniculus | |
12 | - |
ITP | MgITP2-, hexokinase Ia | Oryctolagus cuniculus | |
25 | - |
D-fructose | hexokinase Ib | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | hexokinase Ia and Ib | Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE | Oryctolagus cuniculus |
105000 | - |
reticulocyte hexokinase Ia and Ib, gel filtration, sucrose density gradient sedimentation | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
hexokinase Ia and Ib | - |
Purification (Comment) | Organism |
---|---|
erythrocyte enzyme and reticulocyte hexokinase Ia and Ib | Oryctolagus cuniculus |
hexokinase Ia: 55460fold, hexokinase Ib: 51260fold | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
reticulocyte | hexokinase Ia and Ib | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
144 | - |
pH 8.1 | Oryctolagus cuniculus |
144 | - |
reticulocyte, hexokinase Ib, at 37°C | Oryctolagus cuniculus |
155 | - |
pH 8.1 | Oryctolagus cuniculus |
155 | - |
reticulocyte, hexokinase Ia, at 37°C | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-25°C, over a period of months, stable | Oryctolagus cuniculus |
37°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ib is more stable than hexokinase Ia | Oryctolagus cuniculus |
4°C, sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, several days, hexokinase Ia is more stable than hexokinase Ib | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | hexokinase Ia: 65% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose | Oryctolagus cuniculus | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
CTP + D-glucose | - |
Oryctolagus cuniculus | CDP + D-glucose 6-phosphate | - |
? | |
D-fructose + ATP | hexokinase Ia: 69% of the activity with glucose, hexokinase Ib: 42% of the activity with glucose | Oryctolagus cuniculus | ADP + D-fructose 6-phosphate | - |
? | |
D-glucosamine + ATP | hexokinase Ia: 35% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose | Oryctolagus cuniculus | ADP + D-glucosamine 6-phosphate | - |
? | |
D-glucose + ATP | MgATP2- | Oryctolagus cuniculus | ADP + D-glucose 6-phosphate | - |
? | |
D-mannose + ATP | 104-105% of the activity with glucose | Oryctolagus cuniculus | ADP + D-mannose 6-phosphate | - |
? | |
ITP + D-glucose | 30% of the activity with ATP | Oryctolagus cuniculus | IDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | MgITP | Oryctolagus cuniculus | IDP + D-glucose 6-phosphate | - |
? | |
additional information | not: UTP | Oryctolagus cuniculus | ? | - |
? | |
additional information | not: N-acetyl-D-glucosamine | Oryctolagus cuniculus | ? | - |
? | |
additional information | not: D-galactose | Oryctolagus cuniculus | ? | - |
? | |
additional information | not: GTP | Oryctolagus cuniculus | ? | - |
? | |
additional information | not: CTP | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.2 | - |
Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryctolagus cuniculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryctolagus cuniculus | reticulocyte hexokinase Ib | - |
5.7 |
Oryctolagus cuniculus | isoelectric focusing | - |
5.7 |
Oryctolagus cuniculus | hexokinase Ia, isoelectric focusing | - |
6.2 |