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Literature summary for 2.7.1.1 extracted from
- Multiple forms of Drosophila hexokinase. Purification, biochemical and immunological characterization (1980), J. Biol. Chem., 255, 4673-4679.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Hex A and Hex B are products of the same structural gene, but differ by epigenetic or posttranslational modifications, the gene is mapped on the X chromosome, Hex C is mapped on the second chromosome, Hex C1 and C2 are genetic variants | Drosophila melanogaster |
General Stability
| General Stability | Organism |
|---|---|
| ATP, 4 mM, no protection against thermal inactivation | Drosophila melanogaster |
| D-glucose protects against inactivation at higher temperatures | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Drosophila melanogaster |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | 50000 | gel filtration | Drosophila melanogaster |
| 40000 | 50000 | Hex A, Hex B and Hex C | Drosophila melanogaster |
| 42000 | - |
Hex C2, gel filtration | Drosophila melanogaster |
| 42000 | - |
isoenzyme Hex C2, gel filtration | Drosophila melanogaster |
| 47000 | - |
Hex A, gel filtration | Drosophila melanogaster |
| 47000 | - |
isoenzyme Hex A, gel filtration | Drosophila melanogaster |
| 48000 | - |
Hex B2, gel filtration | Drosophila melanogaster |
| 48000 | - |
isoenzyme Hex B2, gel filtration | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
Samarkand, adult | - |
| Drosophila melanogaster | - |
Hex A and Hex C | - |
| Drosophila melanogaster Samarkand | - |
Samarkand, adult | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of 2 enzyme forms: Hex A: 1370fold, Hex C2: 600fold | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | Hex B predominates in larval extract, Hex A is completely absent | Drosophila melanogaster | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
enzyme form Hex C, at 25°C, pH 8 | Drosophila melanogaster |
| 137 | - |
enzyme form Hex A, at 25°C, pH 8 | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-dATP + D-glucose | 73% of the activity with ATP | Drosophila melanogaster | 2'-dADP + D-glucose 6-phosphate | - |
? |  |
| 2'-dATP + D-glucose | Hex A: 73% of the activity with ATP, Hex B: 76% of the activity with ATP, Hex C: 80% of the activity with ATP | Drosophila melanogaster | 2'-dADP + D-glucose 6-phosphate | - |
? | |
| ATP + 2-deoxy-D-glucose | Hex A: 93% of the activity with glucose, Hex B: 51% of the activity with glucose, Hex C: 48% of the activity with glucose | Drosophila melanogaster | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| ATP + 2-deoxy-D-glucose | 93% of the activity with glucose | Drosophila melanogaster | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| ATP + 2-deoxy-D-glucose | Hex A: 93% of the activity with glucose, Hex B: 51% of the activity with glucose, Hex C: 48% of the activity with glucose | Drosophila melanogaster Samarkand | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| CTP + D-glucose | Hex B: 5% of the activity with ATP, Hex C: 11% of the activity with ATP, not Hex A | Drosophila melanogaster | CDP + D-glucose 6-phosphate | - |
? | |
| D-fructose + ATP | Hex A: 50% of the activity with glucose, Hex B: 41% of the activity with glucose, Hex C: 134% of the activity with glucose | Drosophila melanogaster | ADP + D-fructose 6-phosphate | - |
? | |
| D-fructose + ATP | 50% of the activity with glucose | Drosophila melanogaster | ADP + D-fructose 6-phosphate | - |
? | |
| D-fructose + ATP | Hex A: 50% of the activity with glucose, Hex B: 41% of the activity with glucose, Hex C: 134% of the activity with glucose | Drosophila melanogaster Samarkand | ADP + D-fructose 6-phosphate | - |
? | |
| D-fructose + ATP | 50% of the activity with glucose | Drosophila melanogaster Samarkand | ADP + D-fructose 6-phosphate | - |
? | |
| D-glucosamine + ATP | Hex A: 19% of the activity with glucose, Hex C: 44% of the activity with glucose, not Hex B | Drosophila melanogaster | ADP + D-glucosamine 6-phosphate | - |
? | |
| D-glucose + ATP | - |
Drosophila melanogaster | ADP + D-glucose 6-phosphate | - |
? | |
| D-glucose + ATP | - |
Drosophila melanogaster Samarkand | ADP + D-glucose 6-phosphate | - |
? | |
| D-mannose + ATP | Hex A: 100% of the activity with glucose, Hex B: 98% of the activity with glucose, Hex C: 44% of the activity with glucose | Drosophila melanogaster | ADP + D-mannose 6-phosphate | - |
? | |
| D-mannose + ATP | Hex A: 100% of the activity with glucose, Hex B: 98% of the activity with glucose, Hex C: 44% of the activity with glucose | Drosophila melanogaster Samarkand | ADP + D-mannose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, isoenzyme Hex A, SDS-PAGE | Drosophila melanogaster |
| monomer | 1 * 42000, isoenzyme Hex C2, SDS-PAGE | Drosophila melanogaster |
| monomer | 1 * 48000, isoenzyme Hex B2, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Drosophila melanogaster |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
glucose protects against inactivation at higher temperatures | Drosophila melanogaster |
| 54 | - |
in 0.1 M Tris-HCl, pH 8, all 3 isoforms are inactivated in a time-dependent manner, Hex A and Hex B are more stable than Hex C, glucose protects against thermal inactivation, but not 4 mM ATP | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Drosophila melanogaster |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | activity increases more than 10fold over the range of pH 5.5-7.5, after which the activity remains constant until pH 9 | Drosophila melanogaster |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Drosophila melanogaster | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Drosophila melanogaster | Hex A, isoelectric focusing | - |
4.9 |
| Drosophila melanogaster | Hex B1, isoelectric focusing | - |
5.15 |
| Drosophila melanogaster | Hex B2, isoelectric focusing | - |
5.3 |
| Drosophila melanogaster | Hex C1, isoelectric focusing | - |
6.1 |
| Drosophila melanogaster | Hex C2, isoelectric focusing | - |
6.4 |
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