Cloned (Comment) | Organism |
---|---|
a synthetic gene, encoding hexokinase I from brain, is expressed in Escherichia coli BL21(DE3) under the control of the bacteriophage T7 promoter | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
human enzyme expressed in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-phosphate | hexokinase I inhibition is relieved by inorganic phosphate, but not the inhibition of hexokinase II | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
D-glucose | recombinant hexokinase I expressed in Escherichia coli | Homo sapiens | |
0.3 | - |
ATP | recombinant hexokinase I expressed in Escherichia coli | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
recombinant hexokinase I expressed in Escherichia coli, deduced from nucleotide sequence of the expressed cDNA and gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Homo sapiens | hexokinase I is a regulated enzyme and serves a critical role in controlling the rate of glycolysis in both brain and red blood cells | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant hexokinase I, expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
13fold | Homo sapiens |
recombinant hexokinase I expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58.2 | - |
recombinant hexokinase I expressed in E. coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | hexokinase I is a regulated enzyme and serves a critical role in controlling the rate of glycolysis in both brain and red blood cells | Homo sapiens | D-glucose 6-phosphate + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, recombinant hexokinase I expressed in Escherichia coli, SDS-PAGE | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |