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Literature summary for 2.7.1.1 extracted from
- Hexokinase type II from rat skeletal muscle (1982), Methods Enzymol., 90, 11-15.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| hexokinase type II is extremely unstable, hexose or thiol stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Rattus norvegicus |  |
| D-glucose 1,6-diphosphate | - |
Rattus norvegicus | |
| D-glucose 6-phosphate | - |
Rattus norvegicus | |
| phosphate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
D-glucose | at 30°C, pH 7.5 | Rattus norvegicus | |
| 0.06 | - |
D-mannose | at 30°C, pH 7.5 | Rattus norvegicus | |
| 0.06 | - |
D-glucosamine | at 30°C, pH 7.5 | Rattus norvegicus | |
| 0.5 | - |
ATP | at 30°C, pH 7.5 | Rattus norvegicus | |
| 0.9 | - |
D-fructose | at 30°C, pH 7.5 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | hexokinase type II, largely soluble | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
sedimentation equilibrium | Rattus norvegicus |
| 106000 | - |
gel filtration | Rattus norvegicus |
| 108000 | - |
1 * 108000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar rats | - |
| Rattus norvegicus | - |
hexokinase type II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hexokinase type II, 5330fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | hexokinase type II, best source of enzyme | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 120 | - |
hexokinase type II, at 30°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + ATP | - |
Rattus norvegicus | ADP + D-fructose 6-phosphate | - |
? | |
| D-glucosamine + ATP | - |
Rattus norvegicus | ADP + D-glucosamine 6-phosphate | - |
? | |
| D-glucose + ATP | - |
Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
| D-mannose + ATP | - |
Rattus norvegicus | ADP + D-mannose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 108000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
D-glucose 6-phosphate | ATP | Rattus norvegicus | |
| 0.026 | - |
D-glucose 6-phosphate | hexose | Rattus norvegicus | |
| 3 | - |
D-Glucose 1,6-bisphosphate | - |
Rattus norvegicus | |
| 3.5 | - |
ADP | - |
Rattus norvegicus | |
| 5.3 | - |
phosphate | - |
Rattus norvegicus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rattus norvegicus | analytical isoelectric focusing | - |
5.7 |
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