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Literature summary for 2.7.1.1 extracted from

  • Tsai, C.S.; Chen, Q.
    Purification and kinetic characterization of hexokinase and glucose-6-phosphate dehydrogenase from Schizosaccharomyces pombe (1998), Biochem. Cell Biol., 76, 107-113.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP noncompetitive to glucose, competitive to ATP Schizosaccharomyces pombe
Mg2+ at 10 mM, slight Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation is associated with an increase in the maximal velocity by its interaction with the ternary complex to facilitate the transfer of the phosphoryl group Schizosaccharomyces pombe
Mg2+ strict requirement for divalent metal ions Schizosaccharomyces pombe
Mg2+ maximal activity at 1 mM Mg2+, can be partially replaced by Mn2+ Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
2 * 47500, SDS-PAGE Schizosaccharomyces pombe
88000
-
gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Schizosaccharomyces pombe enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway D-glucose 6-phosphate + ADP
-
?
D-glucose + ATP Schizosaccharomyces pombe NCYC 132S2-2 enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway D-glucose 6-phosphate + ADP
-
?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
2 isozymic forms
-
Schizosaccharomyces pombe NCYC 132S2-2
-
2 isozymic forms
-

Purification (Commentary)

Purification (Comment) Organism
253fold, major isoenzyme form Schizosaccharomyces pombe

Reaction

Reaction Comment Organism Reaction ID
ATP + D-hexose = ADP + D-hexose 6-phosphate equilibrium random Bi Bi mechanism with formation of the abortive enzyme-ADP-glucose complex Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.25
-
at pH 7.2 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ATP
-
Schizosaccharomyces pombe ADP + D-glucose 6-phosphate
-
?
D-glucose + ATP
-
Schizosaccharomyces pombe NCYC 132S2-2 ADP + D-glucose 6-phosphate
-
?
D-glucose + ATP enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway Schizosaccharomyces pombe D-glucose 6-phosphate + ADP
-
?
D-glucose + ATP enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway Schizosaccharomyces pombe NCYC 132S2-2 D-glucose 6-phosphate + ADP
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47500, SDS-PAGE Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
ATP
-
Schizosaccharomyces pombe

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Schizosaccharomyces pombe