Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces lavendulae |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutant K43A, to 2.25 and 1.9 A resolution, respectively. Each monomer of DcsD takes a typical fold of type II pyridoxal 5'-phosphate enzymes with the cofactor pyridoxal 5'-phosphate covalently bound to invariant Lys residue (Lys43) at the active site. The pyridine ring of pyridoxal 5'-phoshate makes hydrogen bonds with invariant Asn73 and Ser265 residues. Its phosphate group makes hydrogen bonds with Gly177, Thr178, Thr179 and Thr181 residues | Streptomyces lavendulae |
Protein Variants | Comment | Organism |
---|---|---|
K43A | mutation in invariant Lys43 residue, inactive. Mutant forms an external aldimine adduct upon addition of L-methionine or O-ureido-L-serine | Streptomyces lavendulae |
S121A | decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine | Streptomyces lavendulae |
S121M | decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine | Streptomyces lavendulae |
V74T | decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine | Streptomyces lavendulae |
Y97F | decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine | Streptomyces lavendulae |
Y97M | decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine | Streptomyces lavendulae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
Hydroxyurea | wild-type, pH 8.0, 30°C | Streptomyces lavendulae | |
18 | - |
Hydroxyurea | mutant Y97F, pH 8.0, 30°C | Streptomyces lavendulae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | D2Z027 | - |
- |
Streptomyces lavendulae ATCC 11924 | D2Z027 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47. The kcat/Km value of DcsD for L-cysteine synthesis is 80fold higher than that for O-ureido-L-serine synthesis | Streptomyces lavendulae | ? | - |
? | |
additional information | enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47. The kcat/Km value of DcsD for L-cysteine synthesis is 80fold higher than that for O-ureido-L-serine synthesis | Streptomyces lavendulae ATCC 11924 | ? | - |
? | |
O-acetyl-L-serine + hydroxyurea | - |
Streptomyces lavendulae | O-ureido-L-serine + acetate | - |
? | |
O-acetyl-L-serine + hydroxyurea | - |
Streptomyces lavendulae ATCC 11924 | O-ureido-L-serine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | both in solution and in a crystalline state, crystallization data | Streptomyces lavendulae |
Synonyms | Comment | Organism |
---|---|---|
DcsD | - |
Streptomyces lavendulae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
Hydroxyurea | mutant Y97F, pH 8.0, 30°C | Streptomyces lavendulae | |
11 | - |
Hydroxyurea | wild-type, pH 8.0, 30°C | Streptomyces lavendulae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Streptomyces lavendulae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Hydroxyurea | mutant S121M, pH 8.0, 30°C | Streptomyces lavendulae | |
0.088 | - |
Hydroxyurea | mutant Y97M, pH 8.0, 30°C | Streptomyces lavendulae | |
0.094 | - |
Hydroxyurea | mutant S121A, pH 8.0, 30°C | Streptomyces lavendulae | |
0.22 | - |
Hydroxyurea | mutant V74T, pH 8.0, 30°C | Streptomyces lavendulae | |
0.25 | - |
Hydroxyurea | mutant Y97F, pH 8.0, 30°C | Streptomyces lavendulae | |
1.2 | - |
Hydroxyurea | wild-type, pH 8.0, 30°C | Streptomyces lavendulae |