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Literature summary for 2.6.99.1 extracted from
- DNA binding activity from cultured human fibrolasts that is specific for partially depurinated DNA and that inserts purines into apurinic sites (1979), Proc. Natl. Acad. Sci. USA, 76, 141-144.
General Stability
| General Stability | Organism |
|---|---|
| sensitive to freezing and heating, extremely sensitive to dialysis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Caffeine | 20-80% inhibition when present at 0.1-1 mM | Homo sapiens |  |
| EDTA | complete inhibition at 1.3 mM | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | specific requirement, cannot be replaced by NH4+, Na+, Mg2+ or Mn2+ | Homo sapiens | |
| Li+ | supports activity to a small degree | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
glycerol density gradient centrifugation | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | possible alternative pathway for DNA repair | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using DEAE-cellulose and phosphocellulose chromatography followed by glycerol gradient centrifugation | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | cultured | Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| unstable, activity retained for less than 1 week after purification, addition of phenylmethylsulfonyl fluoride or N-alpha-p-tosyl-L-lysine chloromethylketone hydrochloride can stabilize enzymatic fractions | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dATP + depurinated DNA | closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate | Homo sapiens | deoxyribose triphosphate + purinated DNA | - |
? | |
| dATP + depurinated poly(dA-dT) | dGTP cannot act as substrate | Homo sapiens | deoxyribose triphosphate + purinated poly(dA-dT) | - |
? | |
| dGTP + depurinated DNA | closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate | Homo sapiens | deoxyribose triphosphate + purinated DNA | - |
? | |
| dGTP + depurinated poly(dG-dC) | dATP cannot act as substrate | Homo sapiens | deoxyribose triphosphate + purinated poly(dG-dC) | - |
? | |
| additional information | specific insertion of adenine into adenine apurinic sites and guanine into guanine apurinic sites | Homo sapiens | ? | - |
? | |
| additional information | possible alternative pathway for DNA repair | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
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Release 2023.1 (January 2023)
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