Literature summary for 2.6.1.B3 extracted from

Hartinger, D.; Heinl, S.; Schwartz, H.E.; Grabherr, R.; Schatzmayr, G.; Haltrich, D.; Moll, W.D.
Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1 (2010), Microb. Cell Fact., 9, 62.

Search for more literature for 2.6.1.B3

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged protein and maltose binding protein fusion protein expressed in different Escherichia coli (DE3) strains at different temeratures and different expression levels	Sphingopyxis sp.

Organism

Organism	UniProt	Comment	Textmining
Sphingopyxis sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), maltose binding protein fusion protein: affinity chromatography (dextrin sepharose), cleavage of the fusion protein	Sphingopyxis sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + pyruvate	-	Sphingopyxis sp.	(3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-alanine	-	?

Synonyms

Synonyms	Comment	Organism
aminotransferase for deamination of hydrolyzed fumonisin B1 (gene fumI)	-	Sphingopyxis sp.
FumI	-	Sphingopyxis sp.

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Sphingopyxis sp.

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Release 2023.1 (January 2023)