Literature summary for 2.6.1.B3 extracted from

Hartinger, D.; Schwartz, H.; Hametner, C.; Schatzmayr, G.; Haltrich, D.; Moll, W.D.
Enzyme characteristics of aminotransferase FumI of Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1 (2011), Appl. Microbiol. Biotechnol., 91, 757-768.

Search for more literature for 2.6.1.B3

Activating Compound

Activating Compound	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Sphingopyxis sp.

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged protein expressed in Escherichia coli ArcticExpress (DE3), His-tag does not affect the enzymatic activity	Sphingopyxis sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol	substrate inhibition	Sphingopyxis sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0011	-	(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol	pH 8.0, 35°C	Sphingopyxis sp.
0.49	-	pyruvate	pH 8.0, 35°C	Sphingopyxis sp.
4.15	-	oxaloacetate	pH 8.0, 35°C	Sphingopyxis sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48270	-	calculated	Sphingopyxis sp.

Organism

Organism	UniProt	Comment	Textmining
Sphingopyxis sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+)	Sphingopyxis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.2	-	pH 8.0, 35°C	Sphingopyxis sp.

Storage Stability

Storage Stability	Organism
-20°C, 25% glycerol, stable for several month	Sphingopyxis sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + oxaloacetate	-	Sphingopyxis sp.	(3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-aspartate	-	?
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + pyruvate	activity observed with glyoxylate and 2-oxobutyrate, no activity detectable with 2-oxogluarate, substrates hydrolyzed fumonisin B2 and B3 also used	Sphingopyxis sp.	(3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-alanine	-	?

Synonyms

Synonyms	Comment	Organism
aminotransferase FumI	-	Sphingopyxis sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Sphingopyxis sp.

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
6	45	-	Sphingopyxis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	rapid inactivation at 50°C	Sphingopyxis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.73	-	(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol	pH 8.0, 35°C	Sphingopyxis sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.25	-	sharp optimum at pH 8.0 and 8.5	Sphingopyxis sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	9.7	-	Sphingopyxis sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	activity reduced at pH 6.5 or less (1 h, 35°C), activity reduced at pH 8.0 (1 h)	Sphingopyxis sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)