Cloned (Comment) | Organism |
---|---|
gene wbpE, expression of His6-tagged WbpE in Escherichia coli strain Rosetta(DE3) | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate | Pseudomonas aeruginosa | transaminase WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
serotype O5, gene wbpE | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged WbpE from Escherichia coli strain Rosetta(DE3) | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, purified recombinant His6-tagged WbpE, 3 weeks, stable | Pseudomonas aeruginosa |
precipitation occurs if the protein is left at ambient temperature or subjected to excessive freeze/thaw cycles | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid WbpE product identification by mass spectrometry, overview | Pseudomonas aeruginosa | ? | - |
? | |
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate | transaminase WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | Pseudomonas aeruginosa | UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate | - |
r | |
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate | transaminase WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate. Cofactor pyridoxal 5'-phosphate is converted to pyridoxamine phosphate during the reaction | Pseudomonas aeruginosa | UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
WbpE | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | WbpE substrate 2-acetamido-2-deoxy-D-ribohex-3-uluronic acid is synthesized by WbpB | Pseudomonas aeruginosa |