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Literature summary for 2.6.1.9 extracted from

  • Weigent, D.A.; Nester, E.W.
    Purification and properties of two aromatic aminotransferases in Bacillus subtilis (1976), J. Biol. Chem., 251, 6974-6980.
    View publication on PubMed
Search for more literature for 2.6.1.9

Inhibitors

Inhibitors Comment Organism Structure
L-glutamate competitive to tyrosine Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 L-histidinol phosphate
-
 Bacillus subtilis
0.71
-
 L-tyrosine
-
 Bacillus subtilis
2.5
-
 L-phenylalanine
-
 Bacillus subtilis
7.1
-
 2-oxoglutarate
-
 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
220fold Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate ping-pong bi bi mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
325
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
0°C, 1 month, 30% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidinol phosphate + 2-oxoglutarate
-
 Bacillus subtilis 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
 ?
L-phenylalanine + 2-oxoglutarate
-
 Bacillus subtilis phenylpyruvate + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Bacillus subtilis 3-indole-2-oxopropanoate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Bacillus subtilis 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 4 min, more than 90% loss of activity, 10 min, in presence of pyridoxal 5'-phosphate, 75% loss of activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 stable overnight Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal phosphate protein Bacillus subtilis
pyridoxal 5'-phosphate 0.0022 mM Bacillus subtilis