Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-nitrotyrosine | approximately 20% inhibition at 1 mM | Klebsiella pneumoniae | |
Canaline | approximately 40% inhibition at 1 mM | Klebsiella pneumoniae | |
carboxymethoxylamine | complete inhibition at 1 mM | Klebsiella pneumoniae | |
malate | approximately 20% inhibition at 1 mM | Klebsiella pneumoniae | |
additional information | serine-O-sulfate and nitrophenylalanine have no inhibitory activity at 0.1 or 1 mM | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.42 | - |
L-tryptophan | recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C | Klebsiella pneumoniae | |
2.01 | - |
L-phenylalanine | recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C | Klebsiella pneumoniae | |
2.01 | - |
L-tyrosine | recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C | Klebsiella pneumoniae | |
2.46 | - |
2-oxo-4-methylthiobutanoate | recombinant enzyme, with L-tyrosine as cosubstrate, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C | Klebsiella pneumoniae | |
11.93 | - |
L-glutamate | recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Klebsiella pneumoniae |
90000 | - |
gel filtration | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae ATCC 13883 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, Red-120 Sepharose column chromatography, Mono-Q column chromatography, S200-Sephacryl gel filtration, and phenylethyl column chromatography | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00314 | - |
crude recombinant enzyme, after incubation with 2 mM each amino acid (ADEFGHIKLNQRSTWY) and 1 mM 2-oxo-4-methylthiobutanoate in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C | Klebsiella pneumoniae |
0.7227 | - |
recombinant enzyme after 722.7fold purification, after incubation with 2 mM each amino acid (ADEFGHIKLNQRSTWY) and 1 mM 2-oxo-4-methylthiobutanoate in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-methylthiobutanoate + an L-amino acid | a wide range of L-amino acids are effective donors, with Asp, Glu, Phe, His, Ile, Leu, Asn, Gln, Trp, and Tyr all producing more than 0.5 nM of L-methionine/min/mg of protein. Glu, Phe, Trp, and Tyr are the preferred amino donors, catalyzing the formation of above 1100 nM L-methionine/min/mg of protein | Klebsiella pneumoniae | L-methionine + a 2-oxo acid | - |
? | |
2-oxo-4-methylthiobutanoate + an L-amino acid | a wide range of L-amino acids are effective donors, with Asp, Glu, Phe, His, Ile, Leu, Asn, Gln, Trp, and Tyr all producing more than 0.5 nM of L-methionine/min/mg of protein. Glu, Phe, Trp, and Tyr are the preferred amino donors, catalyzing the formation of above 1100 nM L-methionine/min/mg of protein | Klebsiella pneumoniae ATCC 13883 | L-methionine + a 2-oxo acid | - |
? | |
2-oxo-4-methylthiobutanoate + L-glutamate | while L-glutamate is capable of producing the highest maximum initial velocity for L-methionine production from 2-oxo-4-methylthiobutanoate, it is 5-10fold poorer in terms of substrate specificity when compared with L-tyrosine, L-tryptophan, and L-phenylalanine | Klebsiella pneumoniae | L-methionine + 2-oxoglutarate | - |
? | |
2-oxo-4-methylthiobutanoate + L-glutamate | while L-glutamate is capable of producing the highest maximum initial velocity for L-methionine production from 2-oxo-4-methylthiobutanoate, it is 5-10fold poorer in terms of substrate specificity when compared with L-tyrosine, L-tryptophan, and L-phenylalanine | Klebsiella pneumoniae ATCC 13883 | L-methionine + 2-oxoglutarate | - |
? | |
2-oxo-4-methylthiobutanoate + L-phenylalanine | - |
Klebsiella pneumoniae | L-methionine + 2-oxo-3-phenylpropanoate | - |
? | |
2-oxo-4-methylthiobutanoate + L-phenylalanine | - |
Klebsiella pneumoniae ATCC 13883 | L-methionine + 2-oxo-3-phenylpropanoate | - |
? | |
2-oxo-4-methylthiobutanoate + L-tryptophan | - |
Klebsiella pneumoniae | L-methionine + 3-(1H-indol-3-yl)-2-oxopropanoate | - |
? | |
2-oxo-4-methylthiobutanoate + L-tryptophan | - |
Klebsiella pneumoniae ATCC 13883 | L-methionine + 3-(1H-indol-3-yl)-2-oxopropanoate | - |
? | |
2-oxo-4-methylthiobutanoate + L-tyrosine | - |
Klebsiella pneumoniae | L-methionine + 4-hydroxyhenylpyruvate | - |
r | |
L-methionine + 2-oxoglutarate | - |
Klebsiella pneumoniae | 2-oxo-4-methylthiobutanoate + L-glutamate | - |
r | |
additional information | the purified enzyme is unable to effectively utilize systems containing L-glutamate and oxaloacetate, L-glutamate and pyruvate, or L-alanine and 2-oxoglutarate | Klebsiella pneumoniae | ? | - |
? | |
additional information | the purified enzyme is unable to effectively utilize systems containing L-glutamate and oxaloacetate, L-glutamate and pyruvate, or L-alanine and 2-oxoglutarate | Klebsiella pneumoniae ATCC 13883 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
KMAT | - |
Klebsiella pneumoniae |
TyrAT | - |
Klebsiella pneumoniae |
tyrosine aminotransferase | - |
Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Klebsiella pneumoniae |