Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Canaline | about 50% residual activity at 1 mM | Mycobacterium tuberculosis | |
carboxymethoxylamine | mixed-type inhibition, about 7% residual activity at 1 mM | Mycobacterium tuberculosis | |
hydroxylamine-O-sulfonic acid | about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
additional information | not inhibited by O-trimethylsilylhydroxylamine | Mycobacterium tuberculosis | |
O-allylhydroxylamine | mixed-type inhibition, about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
O-Benzylhydroxylamine | mixed-type inhibition, about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
O-ethylhydroxylamine | about 20% residual activity at 1 mM | Mycobacterium tuberculosis | |
O-nitrobenzylhydroxylamine | about 30% residual activity at 1 mM | Mycobacterium tuberculosis | |
O-pentafluorobenzylhydroxylamine | about 30% residual activity at 1 mM | Mycobacterium tuberculosis | |
O-tert-butyldimethylsilylhydroxylamine | about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
O-tert-butylhydroxylamine | mixed-type inhibition, about 7% residual activity at 1 mM | Mycobacterium tuberculosis | |
O-tetrahydropyranylhydroxylamine | about 20% residual activity at 1 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.77 | - |
L-valine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.5 | - |
L-leucine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.85 | - |
L-isoleucine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
4.2 | - |
2-oxo-4-methylthiobutanoate | apparent Km value, with L-leucine as cosubstrate, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
7.44 | - |
L-phenylalanine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
9.53 | - |
L-glutamate | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-methylthiobutanoate + an L-amino acid | isoleucine, leucine, and valine are the most effective substrates while glutamate and phenylalanine are also active as amino donors. Tyrosine and tryptophan have a much lesser ability to transaminate 2-oxo-4-methylthiobutanoate and all other L-amino acids are inactive | Mycobacterium tuberculosis | L-methionine + a 2-oxo acid | - |
? | |
2-oxo-4-methylthiobutanoate + L-glutamate | - |
Mycobacterium tuberculosis | L-methionine + ? | - |
? | |
2-oxo-4-methylthiobutanoate + L-isoleucine | - |
Mycobacterium tuberculosis | L-methionine + 2-oxo-methylvalerate | - |
? | |
2-oxo-4-methylthiobutanoate + L-leucine | - |
Mycobacterium tuberculosis | L-methionine + 2-oxo-isocaproate | - |
r | |
2-oxo-4-methylthiobutanoate + L-phenylalanine | - |
Mycobacterium tuberculosis | L-methionine + ? | - |
? | |
2-oxo-4-methylthiobutanoate + L-valine | - |
Mycobacterium tuberculosis | L-methionine + 2-oxo-isovalerate | - |
? | |
L-methionine + 2-oxo-isocaproate | - |
Mycobacterium tuberculosis | 2-oxo-4-methylthiobutanoate + L-leucine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Mycobacterium tuberculosis |
branched-chain amino acid aminotransferase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
O-Benzylhydroxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.01102 | - |
O-tert-butylhydroxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.02097 | - |
carboxymethoxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.02161 | - |
O-allylhydroxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.08408 | - |
O-Benzylhydroxylamine | uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.0856 | - |
O-tert-butylhydroxylamine | uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.1424 | - |
carboxymethoxylamine | uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.2 | - |
O-allylhydroxylamine | Ki above 0.2 mM, uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis |