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Literature summary for 2.6.1.88 extracted from

  • Venos, E.S.; Knodel, M.H.; Radford, C.L.; Berger, B.J.
    Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis (2004), BMC Microbiol., 4, 39.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Canaline about 50% residual activity at 1 mM Mycobacterium tuberculosis
carboxymethoxylamine mixed-type inhibition, about 7% residual activity at 1 mM Mycobacterium tuberculosis
hydroxylamine-O-sulfonic acid about 10% residual activity at 1 mM Mycobacterium tuberculosis
additional information not inhibited by O-trimethylsilylhydroxylamine Mycobacterium tuberculosis
O-allylhydroxylamine mixed-type inhibition, about 10% residual activity at 1 mM Mycobacterium tuberculosis
O-Benzylhydroxylamine mixed-type inhibition, about 10% residual activity at 1 mM Mycobacterium tuberculosis
O-ethylhydroxylamine about 20% residual activity at 1 mM Mycobacterium tuberculosis
O-nitrobenzylhydroxylamine about 30% residual activity at 1 mM Mycobacterium tuberculosis
O-pentafluorobenzylhydroxylamine about 30% residual activity at 1 mM Mycobacterium tuberculosis
O-tert-butyldimethylsilylhydroxylamine about 10% residual activity at 1 mM Mycobacterium tuberculosis
O-tert-butylhydroxylamine mixed-type inhibition, about 7% residual activity at 1 mM Mycobacterium tuberculosis
O-tetrahydropyranylhydroxylamine about 20% residual activity at 1 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.77
-
L-valine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
2.5
-
L-leucine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
2.85
-
L-isoleucine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
4.2
-
2-oxo-4-methylthiobutanoate apparent Km value, with L-leucine as cosubstrate, at pH 7.4, 37°C Mycobacterium tuberculosis
7.44
-
L-phenylalanine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
9.53
-
L-glutamate apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-methylthiobutanoate + an L-amino acid isoleucine, leucine, and valine are the most effective substrates while glutamate and phenylalanine are also active as amino donors. Tyrosine and tryptophan have a much lesser ability to transaminate 2-oxo-4-methylthiobutanoate and all other L-amino acids are inactive Mycobacterium tuberculosis L-methionine + a 2-oxo acid
-
?
2-oxo-4-methylthiobutanoate + L-glutamate
-
Mycobacterium tuberculosis L-methionine + ?
-
?
2-oxo-4-methylthiobutanoate + L-isoleucine
-
Mycobacterium tuberculosis L-methionine + 2-oxo-methylvalerate
-
?
2-oxo-4-methylthiobutanoate + L-leucine
-
Mycobacterium tuberculosis L-methionine + 2-oxo-isocaproate
-
r
2-oxo-4-methylthiobutanoate + L-phenylalanine
-
Mycobacterium tuberculosis L-methionine + ?
-
?
2-oxo-4-methylthiobutanoate + L-valine
-
Mycobacterium tuberculosis L-methionine + 2-oxo-isovalerate
-
?
L-methionine + 2-oxo-isocaproate
-
Mycobacterium tuberculosis 2-oxo-4-methylthiobutanoate + L-leucine
-
r

Synonyms

Synonyms Comment Organism
BcaT
-
Mycobacterium tuberculosis
branched-chain amino acid aminotransferase
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0082
-
O-Benzylhydroxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
0.01102
-
O-tert-butylhydroxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
0.02097
-
carboxymethoxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
0.02161
-
O-allylhydroxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
0.08408
-
O-Benzylhydroxylamine uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
0.0856
-
O-tert-butylhydroxylamine uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
0.1424
-
carboxymethoxylamine uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
0.2
-
O-allylhydroxylamine Ki above 0.2 mM, uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis