Cloned (Comment) | Organism |
---|---|
overexpressed using a T7lac promoter-driven construct, hexahistidine-tagged fusion protein | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate | Escherichia coli | ArnB catalyzes the reversible transfer of the amino group from glutamate to the acceptor, uridine 5'-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate), the intermediate that is synthesized by ArnA from UDP-glucuronic acid.the enzyme is highly selective for glutamate as the amine donor, but the equilibrium constant in the direction of UDP-4-amino-4-deoxy-beta-L-arabinose formation is unfavorable | UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77690 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate | ArnB catalyzes the reversible transfer of the amino group from glutamate to the acceptor, uridine 5'-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate), the intermediate that is synthesized by ArnA from UDP-glucuronic acid.the enzyme is highly selective for glutamate as the amine donor, but the equilibrium constant in the direction of UDP-4-amino-4-deoxy-beta-L-arabinose formation is unfavorable | Escherichia coli | UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate | - |
r | |
UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate | the enzyme is highly selective for glutamate as the amine donor, but the equilibrium constant in the direction of UDP-4-amino-4-deoxy-beta-L-arabinose formation is unfavorable. The rate of transamination with L-methionine, L-glutamine, and L-alanine is measurable at 5%, 2%, and 1%, respectively, of the rate observed with L-glutamate | Escherichia coli | UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | the pyridoxal phosphate is converted to the pyridoxamine form in the presence of excess glutamate | Escherichia coli |