Literature summary for 2.6.1.86 extracted from

Li, Q.A.; Mavrodi, D.V.; Thomashow, L.S.; Roessle, M.; Blankenfeldt, W.
Ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (ADIC) synthase PhzE (2011), J. Biol. Chem., 286, 18213-18221.

Search for more literature for 2.6.1.86

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged protein expressed in Escherichia coli BL21(DE3)	Burkholderia lata

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion method, PEG 3350	Burkholderia lata

Inhibitors

Inhibitors	Comment	Organism	Structure
Mn2+	reduced activity	Burkholderia lata
Ni2+	reduced activity	Burkholderia lata
Zn2+	reduced activity	Burkholderia lata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0024	-	chorismate	pH 7.0, 25°C	Burkholderia lata

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Burkholderia lata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals	Burkholderia lata

Organism

Organism	UniProt	Comment	Textmining
Burkholderia lata	-	-	-
Burkholderia lata 383	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+), cleavage of the tag, gel filtration	Burkholderia lata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + chorismate	-	Burkholderia lata	(S)-2-amino-2-deoxyisochorismate + L-glutamate	-	?
L-glutamine + chorismate	-	Burkholderia lata 383	(S)-2-amino-2-deoxyisochorismate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals	Burkholderia lata

Synonyms

Synonyms	Comment	Organism
2-amino-2desoxyisochorismate synthase	-	Burkholderia lata
PhzE	-	Burkholderia lata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	chorismate	pH 7.0, 25°C	Burkholderia lata

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Release 2023.1 (January 2023)