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Literature summary for 2.6.1.64 extracted from
- Highly purified glutamine transaminase from rat brain. Physical and kinetic properties (1975), Eur. J. Biochem., 58, 153-158.
General Stability
| General Stability | Organism |
|---|---|
| 2-oxo acids protect against heat denaturation | Rattus norvegicus |
| rather unstable in concentrated ammonium sulfate solution | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | 36 mM, 87% inhibition | Rattus norvegicus |  |
| Isonicotinic acid hydrazide | 5 mM, 80% inhibition | Rattus norvegicus | |
| p-chloromercuribenzoate | 0.2 mM, 92% inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
L-glutamine | pH 9.0, 37.5°C | Rattus norvegicus | |
| 1.5 | - |
glyoxylate | pH 9.0, 37.5°C | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Rattus norvegicus |
| 98000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + phenylpyruvate | Rattus norvegicus | - |
2-oxoglutaramate + L-phenylalanine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.65 | - |
brain enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxoglutaramate + L-glutamate | - |
r | |
| L-glutamine + glyoxylate | - |
Rattus norvegicus | 2-oxoglutaramate + glycine | - |
r | |
| L-glutamine + oxaloacetate | - |
Rattus norvegicus | 2-oxoglutaramate + L-aspartate | - |
r | |
| L-glutamine + phenylpyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-phenylalanine | - |
r | |
| L-glutamine + pyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-alanine | - |
r | |
| additional information | no transamination detected with glutamate, aspartate, asparagine, glycine, alanine, 4-aminobutyrate or cysteine as substrate | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable in presence of pyruvate, 90% activity recovered after 30 min, all activity is lost after 15 min in absence of 2-oxoacids | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.2 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | 10 | about half-maximal activity at pH 8.2 and 10.0 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
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